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Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice

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Title: Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice
Authors: Kashiwakura, Jun-ichi Browse this author →KAKEN DB
Yoshihara, Mari Browse this author
Saitoh, Kodai Browse this author
Kagohashi, Kota Browse this author
Sasaki, Yuto Browse this author
Kobayashi, Fuki Browse this author
Inagaki, Iori Browse this author
Kitai, Yuichi Browse this author →KAKEN DB
Muromoto, Ryuta Browse this author →KAKEN DB
Matsuda, Tadashi Browse this author →KAKEN DB
Keywords: Basophils
Food allergy
Issue Date: 2-Dec-2020
Publisher: Japanese Society of Allergology
Journal Title: Allergology international
Volume: 70
Issue: 3
Start Page: 360
End Page: 367
Publisher DOI: 10.1016/j.alit.2020.11.005
Abstract: Background: Propolis is a resinous mixture produced by honey bees that contains cinnamic acid derivatives and flavonoids. Although propolis has been reported to inhibit mast cell functions and mast cell-dependent allergic responses, the effect of propolis on basophil biology remains unknown. This study aimed to investigate the inhibitory effect of propolis on Fc epsilon RI-mediated basophil activation. Methods: To determine the inhibitory effect of propolis on basophil activation in vitro, cytokine production and Fc epsilon RI signal transduction were analyzed by ELISA and western blotting, respectively. To investigate the inhibitory effect of propolis in vivo, IgE-CAI and a food allergy mouse model were employed. Results: Propolis treatment resulted in the suppression of IgE/antigen-induced production of IL-4, IL-6 and IL-13 in basophils. Phosphorylation of Fc epsilon RI signaling molecules Lyn, Akt and ERK was inhibited in basophils treated with propolis. While propolis did not affect the basophil population in the treated mice, propolis did inhibit IgE-CAI. Finally, ovalbumin-induced intestinal anaphylaxis, which involves basophils and basophil-derived IL-4, was attenuated in mice prophylactically treated with propolis. Conclusions: Taken together, these results demonstrate the ability of propolis to suppress IgE-dependent basophil activation and basophil-dependent allergic inflammation. Therefore, prophylactic treatment with propolis may be useful for protection against food allergic reactions in sensitive individuals.
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 柏倉 淳一

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