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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Allocation of Carbon from an Arbuscular Mycorrhizal Fungus, Gigaspora margarita, to Its Gram-Negative and Positive Endobacteria Revealed by High-Resolution Secondary Ion Mass Spectrometry
| Title: | Allocation of Carbon from an Arbuscular Mycorrhizal Fungus, Gigaspora margarita, to Its Gram-Negative and Positive Endobacteria Revealed by High-Resolution Secondary Ion Mass Spectrometry |
| Authors: | Kuga, Yukari Browse this author | | Wu, Ting-Di Browse this author | | Sakamoto, Naoya Browse this author | | Katsuyama, Chie Browse this author | | Yurimoto, Hisayoshi Browse this author →KAKEN DB |
| Keywords: | arbuscular mycorrhiza | | endobacteria | | stable isotope labelling | | secondary ion mass spectrometry |
| Issue Date: | Dec-2021 |
| Publisher: | MDPI |
| Journal Title: | Microorganisms |
| Volume: | 9 |
| Issue: | 12 |
| Start Page: | 2597 |
| Publisher DOI: | 10.3390/microorganisms9122597 |
| Abstract: | Arbuscular mycorrhizal fungi are obligate symbionts of land plants; furthermore, some of the species harbor endobacteria. Although the molecular approach increased our knowledge of the diversity and origin of the endosymbiosis and its metabolic possibilities, experiments to address the functions of the fungal host have been limited. In this study, a C flow of the fungus to the bacteria was investigated. Onion seedlings colonized with Gigaspora margarita, possessing Candidatus Glomeribacter gigasporarum (CaGg, Gram-negative, resides in vacuole) and Candidatus Moeniiplasma glomeromycotorum (CaMg, Gram-positive, resides in the cytoplasm,) were labelled with (CO2)-C-13. The C-13 localization within the mycorrhiza was analyzed using high-resolution secondary ion mass spectrometry (SIMS). Correlative TEM-SIMS analysis of the fungal cells revealed that the C-13/C-12 ratio of CaGg was the lowest among CaMg and mitochondria and was the highest in the cytoplasm. By contrast, the plant cells, mitochondria, plastids, and fungal cytoplasm, which are contributors to the host, showed significantly higher C-13 enrichment than the host cytoplasm. The C allocation patterns implied that CaMg has a greater impact than CaGg on G. margarita, but both seemed to be less burdensome to the host fungus in terms of C cost. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83919 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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