|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Biosynthesis of poly(glycolate-co-3-hydroxybutyrate-co-3-hydroxyhexanoate) in Escherichia coli expressing sequence-regulating polyhydroxyalkanoate synthase and medium-chain-length 3-hydroxyalkanoic acid coenzyme A ligase
| Title: | Biosynthesis of poly(glycolate-co-3-hydroxybutyrate-co-3-hydroxyhexanoate) in Escherichia coli expressing sequence-regulating polyhydroxyalkanoate synthase and medium-chain-length 3-hydroxyalkanoic acid coenzyme A ligase |
| Authors: | Tomita, Hiroya Browse this author →KAKEN DB | | Satoh, Keigo Browse this author | | Nomura, Christopher T Browse this author | | Matsumoto, Ken'ichiro Browse this author →KAKEN DB |
| Keywords: | polyhydroxyalkanoate | | block copolymer | | glycolate | | 3-hydroxyhexanoate |
| Issue Date: | Feb-2022 |
| Publisher: | Oxford University Press |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Volume: | 86 |
| Issue: | 2 |
| Start Page: | 217 |
| End Page: | 223 |
| Publisher DOI: | 10.1093/bbb/zbab198 |
| PMID: | 34788370 |
| Abstract: | Chimeric polyhydroxyalkanoate synthase PhaCAR is characterized by the capacity to incorporate unusual glycolate (GL) units and spontaneously synthesize block copolymers. The GL and 3-hydroxybutyrate (3HB) copolymer synthesized by PhaCAR is a random-homo block copolymer, poly(GL-ran-3HB)-b-poly(3HB). In the present study, medium-chain-length 3-hydroxyhexanoate (3HHx) units were incorporated into this copolymer using PhaCAR for the first time. The coenzyme A (CoA) ligase from Pseudomonas oleovorans (AlkK) serves as a simple 3HHx-CoA supplying route in Escherichia coli from exogenously supplemented 3HHx. NMR analyses of the obtained polymers revealed that 3HHx units were randomly connected to 3HB units, whereas GL units were heterogeneously distributed. Therefore, the polymer is composed of two segments: P(3HB-co-3HHx) and P(GL-co-3HB-co-3HHx). The thermal and mechanical properties of the terpolymer indicate no contiguous P(3HB) segments in the material, consistent with the NMR results. Therefore, PhaCAR synthesized the novel block copolymer P(3HB-co-3HHx)-b-P(GL-co-3HB-co-3HHx), which is the first block PHA copolymer comprising two copolymer segments. |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience biotechnology and biochemistry following peer review. The version of record Volume 86, Issue 2, February 2022, Pages 217–223, is available online at: https://doi.org/10.1093/bbb/zbab198. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/83966 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 冨田 宏矢
|
