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Selection of nuclear genotypes associated with the thermo-sensitivity of Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.)
Title: | Selection of nuclear genotypes associated with the thermo-sensitivity of Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.) |
Authors: | Matsuhira, Hiroaki Browse this author | Kitazaki, Kazuyoshi Browse this author →KAKEN DB | Matsui, Katsunori Browse this author | Kubota, Keisi Browse this author | Kuroda, Yosuke Browse this author | Kubo, Tomohiko Browse this author →KAKEN DB |
Keywords: | Cytoplasmic male sterility | environmental sensitivity | hybrid breeding | restorer of fertility | sugar beet |
Issue Date: | 11-Feb-2022 |
Publisher: | Springer |
Journal Title: | Theoretical and Applied Genetics |
Volume: | 135 |
Start Page: | 1457 |
End Page: | 1466 |
Publisher DOI: | 10.1007/s00122-022-04046-7 |
Abstract: | The stability of cytoplasmic male sterility expression in several genetic backgrounds was investigated in sugar beet (Beta vulgaris L.). Nine genetically heterogenous plants from open-pollinated varieties were crossed with a cytoplasmic male sterile line to obtain 266 F1 plants. Based on marker analysis using a multiallelic DNA marker linked to restorer-of-fertility 1 (Rf1), we divided the F1 plants into 15 genotypes. We evaluated the phenotypes of the F1 plants under two environmental conditions: greenhouse rooms with or without daytime heating during the flowering season. Three phenotypic groups appeared: those consistently expressing male sterility, those consistently having restored pollen fertility, and those expressing male sterility in a thermo-sensitive manner. All plants in the consistently male sterile group inherited a specific Rf1 marker type named p4. We tested the potential for thermo-sensitive male sterile plants to serve as seed parents for hybrid seed production, and three genotypes were selected. Open pollination by a pollen parental line with a dominant trait of red-pigmented hypocotyls and leaf veins resulted in seed setting on thermo-sensitive male sterile plants, indicating that their female organs were functional. More than 99.9% of the progeny expressed the red pigmentation trait; hence, highly pure hybrids were obtained. We determined the nucleotide sequences of Rf1 from the three genotypes: One had a novel allele and two had known alleles, of which one was reported to have been selected previously as a non-restoring allele at a single U.S. breeding station but not at other stations in the U.S., or in Europe or Japan, suggesting environmental sensitivity. |
Description: | メタデータ先行公開 |
Rights: | The final publication is available at link.springer.com. |
Relation: | SharedIt link https://rdcu.be/cGRtw |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/84069 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 久保 友彦
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