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Downregulation of AlphaB-crystallin in Retinal Pigment Epithelial Cells Exposed to Diabetes-related Stimuli In Vivo and In Vitro
| Title: | Downregulation of AlphaB-crystallin in Retinal Pigment Epithelial Cells Exposed to Diabetes-related Stimuli In Vivo and In Vitro |
| Authors: | Wu, Di Browse this author | | Kase, Satoru Browse this author →KAKEN DB | | Liu, Ye Browse this author | | Kanda, Atsuhiro Browse this author | | Murata, Miyuki Browse this author →KAKEN DB | | Ishida, Susumu Browse this author →KAKEN DB |
| Keywords: | AlphaB-crystallin | | retinal pigment epithelial cells | | oxidative stress | | high glucose | | streptozotocin | | advanced glycation end-products |
| Issue Date: | Jan-2022 |
| Publisher: | International Institute of Anticancer Research |
| Journal Title: | In vivo |
| Volume: | 36 |
| Issue: | 1 |
| Start Page: | 132 |
| End Page: | 139 |
| Publisher DOI: | 10.21873/invivo.12684 |
| Abstract: | Background/Aim: AlphaB-crystallin plays a pivotal role in many diseases. However, the involvement of alphaB-crystallin in retinal pigment epithelial (RPE) cells with diabetes stimuli remains unknown. The aim of this study is to examine the alterations of RPE cells and alphaB-crystallin expression in diabetic models in vivo and in vitro. Materials and Methods: Diabetic conditions in mice were induced by streptozotocin (STZ). The thickness of the RPE/choroid complex was measured by optical coherence tomography (OCT). Periodic acid-Schiff (PAS) staining was used to investigate the choriocapillaris in histological sections of murine eyeballs and oxidative stress was evaluated using immunofluorescence with anti-4-hydroxynonenal (HNE) antibody. AlphaB-crystallin expression was examined in the RPE/choroid complex using ELISA. Real-Time PCR was performed to evaluate the alphaB-crystallin expression in cultured human RPE cells with high glucose or following advanced glycation end-products (AGE) stimulation. Results: In diabetic mice, OCT-based RPE/choroidal layers were thickened 2 months after STZ stimulation, where PAS-positive dilated choriocapillaris was noted. Immunoreactivity of 4HNE was strongly observed in the RPE layer, from which a significant number of RPE cells was lost. Meanwhile, alphaBcrystallin expression in 2-month STZ mice was significantly lower compared to controls. In accordance with these results, in vitro data showed that the alphaB-crystallin expression was also significantly lower in RPE cells with high glucose or following AGE stimulation compared to untreated cells. Conclusion: In both types of diabetic models the expression of alphaB-crystallin was found to be downregulated in RPE cells and was associated with increased levels of oxidative stress. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84256 |
| Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加瀬 諭
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