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Visualization of DNA Replication in Single Chromosome by Stable Isotope Labeling

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Title: Visualization of DNA Replication in Single Chromosome by Stable Isotope Labeling
Authors: Nagata, Kosuke Browse this author
Bajo, Ken-ichi Browse this author →KAKEN DB
Mitomo, Hideyuki Browse this author →KAKEN DB
Fujita, Ryosuke Browse this author →KAKEN DB
Uehara, Ryota Browse this author →KAKEN DB
Ijiro, Kuniharu Browse this author →KAKEN DB
Yurimoto, Hisayoshi Browse this author →KAKEN DB
Keywords: stable isotope
chromosome replication
semi-conservative replication
mass spectrometry
Issue Date: 2021
Publisher: 一般社団法人 日本細胞生物学会(Japan Society for Cell Biology)
Journal Title: Cell structure and function
Volume: 46
Issue: 2
Start Page: 95
End Page: 101
Publisher DOI: 10.1247/csf.21011
PMID: 34565768
Abstract: Among the inheritance of cellular components during cell division, deoxyribonucleic acid (DNA) and its condensate (chromosome) are conventionally visualized using chemical tag-labeled nucleotide analogs. However, associated mutagenesis with nucleotide analogs in the visualization of chromosomes is cause for concern. This study investigated the efficiency of using stable isotope labels in visualizing the replicating cultured human cell-chromosomes, in the absence of analog labels, at a high spatial resolution of 100 nm. The distinct carbon isotope ratio between sister chromatids reflected the semi-conservative replication of individual DNA strands through cell cycles and suggested the renewal of histone molecules in daughter chromosomes. Thus, this study provides a new, powerful approach to trace and visualize cellular components with stable isotope labeling.
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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