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Visualization of DNA Replication in Single Chromosome by Stable Isotope Labeling
Title: | Visualization of DNA Replication in Single Chromosome by Stable Isotope Labeling |
Authors: | Nagata, Kosuke Browse this author | Bajo, Ken-ichi Browse this author →KAKEN DB | Mitomo, Hideyuki Browse this author →KAKEN DB | Fujita, Ryosuke Browse this author →KAKEN DB | Uehara, Ryota Browse this author →KAKEN DB | Ijiro, Kuniharu Browse this author →KAKEN DB | Yurimoto, Hisayoshi Browse this author →KAKEN DB |
Keywords: | stable isotope | chromosome replication | semi-conservative replication | imaging | mass spectrometry |
Issue Date: | 2021 |
Publisher: | 一般社団法人 日本細胞生物学会(Japan Society for Cell Biology) |
Journal Title: | Cell structure and function |
Volume: | 46 |
Issue: | 2 |
Start Page: | 95 |
End Page: | 101 |
Publisher DOI: | 10.1247/csf.21011 |
PMID: | 34565768 |
Abstract: | Among the inheritance of cellular components during cell division, deoxyribonucleic acid (DNA) and its condensate (chromosome) are conventionally visualized using chemical tag-labeled nucleotide analogs. However, associated mutagenesis with nucleotide analogs in the visualization of chromosomes is cause for concern. This study investigated the efficiency of using stable isotope labels in visualizing the replicating cultured human cell-chromosomes, in the absence of analog labels, at a high spatial resolution of 100 nm. The distinct carbon isotope ratio between sister chromatids reflected the semi-conservative replication of individual DNA strands through cell cycles and suggested the renewal of histone molecules in daughter chromosomes. Thus, this study provides a new, powerful approach to trace and visualize cellular components with stable isotope labeling. |
Type: | article |
URI: | http://hdl.handle.net/2115/84831 |
Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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