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Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes

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Title: Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes
Authors: Yonamine, Rina Browse this author
Ichihara, Kensuke Browse this author →KAKEN DB
Tsuyuzaki, Shiro Browse this author →KAKEN DB
Hervé, Cécile Browse this author
Motomura, Taizo Browse this author →KAKEN DB
Nagasato, Chikako Browse this author →KAKEN DB
Keywords: cell wall
immunoelectron microscopy
Silvetia babingtonii
transmission electron microscopy
Issue Date: 1-May-2021
Publisher: John Wiley & Sons
Journal Title: Journal of phycology
Volume: 57
Issue: 4
Start Page: 1356
End Page: 1367
Publisher DOI: 10.1111/jpy.13178
Abstract: We examined the ultrastructure of the cell wall and immunolocalization of alginates using specific antibodies against M-rich alginates and MG blocks during rhizoid formation in fucoid zygotes, Silvetia babingtonii. The thallus region of 24-h-old zygotes had a cell wall made of three layers with different fiber distribution. In the 12-h-old zygotes, three layers in the thallus were observed before rhizoid formation, namely the inner, middle, and outer layers. During rhizoid elongation, only the inner layer was apparent close to the rhizoid tip area. Immunoelectron microscopy detected M-rich blocks of alginate on the inner half of the cell wall, irrespective of the number of layers in the thallus and rhizoid regions. The MG blocks were seen to cover a slightly wider area than M-rich alginate blocks. It was suggested that parts of M in mannuronan would be rapidly converted to G, and MG-blocks are generated. Transcriptome analysis was performed using 3 -, 10 -, and 24-h-old zygotes after fertilization to examine the relationship between gene expression and alginate synthesis over time. The expression of two mannuronan C5-epimerase homologs that convert mannuronic acid into guluronic acid in alginates was upregulated or downregulated over the course of the examination.
Rights: This is the peer reviewed version of the following article: Yonamine, R., Ichihara, K., Tsuyuzaki, S., Hervé, C., Motomura, T. and Nagasato, C. (2021), Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes. J. Phycol., which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長里 千香子

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