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Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP

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Title: Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP
Authors: Hata, Shoji Browse this author
Koyama, Suguru Browse this author
Kawahara, Hiroyuki Browse this author
Doi, Naoko Browse this author
Maeda, Tatsuya Browse this author
Toyama-Sorimachi, Noriko Browse this author
Abe, Keiko Browse this author
Suzuki, Koichi Browse this author
Sorimachi, Hiroyuki Browse this author
Keywords: tissue-specific caplain
musscle-specific calpain
cysteine protease
appendage domain
Issue Date: 21-Apr-2006
Journal Title: Journal of biological chemistry
Volume: 281
Issue: 16
Start Page: 11214
End Page: 11224
Publisher DOI: 10.1074/jbc.M509244200
Abstract: Calpain is a Ca2+-regulated cytosolic protease. Mammals have 14 calpain genes, half of which are predominantly expressed in specific organ(s); the rest are expressed ubiquitously. A defect in calpains causes lethality/pathogenicity, indicating their physiological indispensability. nCL-2/calpain-8a was identified as a stomach-specific calpain, whose physiological functions are unclear. To elucidate these, we characterized nCL-2 in detail. Unexpectedly, nCL-2 was localized strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Yeast two-hybrid screening identified several nCL-2-intracting molecules. Of these, the -subunit of coatomer complex (-COP) occurs in the stomach pit cells and is proteolyzed by nCL-2 in vitro. Furthermore, -COP and nCL-2 co-expressed in COS7 cells co-localized in the Golgi, and Ca2+-ionophore stimulation caused the proteolysis of -COP near the linker region, resulting in the dissociation of -COP from the Golgi. These results strongly suggest novel functions for nCL-2 that involve the membrane trafficking of mucus cells via interactions with coat protein.
Rights: Copyright(c)2006 by the American Society for Biochemistry and Molecular Biology
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 裕之

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