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Cholecystokinin secretion induced by β-conglycinin peptone depends on Gαq-mediated pathways in enteroendocrine cells

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Title: Cholecystokinin secretion induced by β-conglycinin peptone depends on Gαq-mediated pathways in enteroendocrine cells
Authors: Hira, Tohru Browse this author →KAKEN DB
Maekawa, Toshihiro Browse this author
Asano, Kozo Browse this author
Hara, Hiroshi Browse this author →KAKEN DB
Keywords: enteroendocrine cells
dietary peptide
Issue Date: 2008
Journal Title: European Journal of Nutrition
Volume: 48
Issue: 2
Start Page: 124
End Page: 127
Publisher DOI: 10.1007/s00394-008-0764-1
Abstract: Background Intraduodenal administration of peptone prepared from soybean β-conglycinin (BconP) stimulates cholecystokinin (CCK) secretion from enteroendocrine cells, and suppresses food intake in rats. However, the sensing mechanism of BconP by CCK-producing cells is unknown. Aim of the study We investigated signal transduction pathways mediating CCK secretion in response to BconP in the murine CCK-producing cell line, STC-1. Methods STC-1 cells were seeded in 48-well culture plates until sub-confluent and CCK secretion was examined under various conditions. CCK concentration was determined by the enzyme immunoassay. Results BconP dose-dependently induced CCK secretion in STC-1 cells. Treatment with BAPTA-AM, an intracellular Ca2+ chelator, reduced BconP-induced CCK secretion, however, removal of extracellular Ca2+ did not affect the secretory response. Treatment with 2-amino borate (2-APB) reduced CCK releasing responses, suggesting the involvement of IP3. In addition, BconP failed to induce CCK secretion after treatment with the Gαq protein inhibitor (YM-254890). Conclusion These results indicate that Gαq pathway is responsible for BconP-induced CCK secretion in STC-1 cells, and suggest the involvement of a Gαq-coupled GPCR(s) in dietary peptide sensing in enteroendocrine cells.
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Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 比良 徹

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