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Soybean β51–63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cells

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Title: Soybean β51–63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cells
Authors: Nakajima, Shingo Browse this author
Hira, Tohru Browse this author →KAKEN DB
Eto, Yuzuru Browse this author
Asano, Kozo Browse this author
Hara, Hiroshi Browse this author →KAKEN DB
Keywords: β51–63 peptide
Enteroendocrine cells
Calcium-sensing receptor
Issue Date: 8-Jan-2010
Journal Title: Regulatory Peptides
Volume: 159
Issue: 1-3
Start Page: 148
End Page: 155
Publisher DOI: 10.1016/j.regpep.2009.11.007
Abstract: We previously demonstrated that intraduodenal administration of an arginine-rich β51-63 peptide in soybean β-conglycinin suppresses food intake via cholecystokinin (CCK) secretion in rats. However, the cellular mechanisms by which the β51-63 peptide induces CCK secretion remain to be clarified. In the present study, we examined whether the extracellular calcium-sensing receptor (CaR) mediates β51-63-induced CCK secretion in murine CCK-producing enteroendocrine cell line STC-1. CCK secretion and changes in intracellular Ca2+ concentration in response to β51-63 peptide were measured in STC-1 cells under various extracellular Ca2+ concentrations and after treatment with a CaR antagonist. Intracellular Ca2+ concentrations in response to β51-63 peptide and extracellular Ca2+ were also measured in CaR-expressing human embryonic kidney (HEK-293) cells. The β51-63 peptide induced CCK secretion and intracellular Ca2+ mobilization in STC-1 cells under normal (1.2 mM) extracellular Ca2+ conditions in a dose-dependent manner. These responses to β51-63 peptide were reduced by the removal of intra- or extracellular Ca2+ but enhanced by increasing extracellular Ca2+ concentrations. Intracellular Ca2+ mobilization induced by extracellular Ca2+ was also increased by the pretreatment with β51-63 peptide. Treatment with a specific CaR antagonist (NPS2143) inhibited β51-63-induced CCK secretion and intracellular Ca2+ mobilization. In addition, HEK-293 cells transfected with CaR acquired sensitivity to the β51-63 peptide. From these results, we conclude that CaR is the β51-63 peptide sensor responsible for the stimulation of CCK secretion in enteroendocrine STC-1 cells.
Rights: (c) 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 比良 徹

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