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Isolation of poly[d-lactate (LA)-co-3-hydroxybutyrate)]-degrading bacteria from soil and characterization of D-LA homo-oligomer degradation by the isolated strains

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Title: Isolation of poly[d-lactate (LA)-co-3-hydroxybutyrate)]-degrading bacteria from soil and characterization of D-LA homo-oligomer degradation by the isolated strains
Authors: Hori, Chiaki Browse this author →KAKEN DB
Sugiyama, Tomohiro Browse this author
Watanabe, Kodai Browse this author
Sun, Jian Browse this author
Kamada, Yuu Browse this author
Ooi, Toshihiko Browse this author →KAKEN DB
Isono, Takuya Browse this author
Satoh, Toshifumi Browse this author →KAKEN DB
Sato, Shin-ichiro Browse this author →KAKEN DB
Taguchi, Seiichi Browse this author →KAKEN DB
Matsumoto, Ken'ichiro Browse this author →KAKEN DB
Keywords: Polyhydroxyalkanoate
Biodegradable plastic
Issue Date: Sep-2020
Publisher: Elsevier
Journal Title: Polymer Degradation and Stability
Volume: 179
Start Page: 109231
Publisher DOI: 10.1016/j.polymdegradstab.2020.109231
Abstract: P[d-lactate (LA)-co-3-hydroxybutyrate (3HB)] is an artificial polyhydroxyalkanoate (PHA) containing unusual D-LA units. In this study, the P(D-LA-co-3HB)-degrading bacterial group in the soil was analyzed and the bacterial degradation of the D-LA clustering structure in the copolymer were evaluated by using chemically synthetic D-LA homo-oligomers. A total of 216 soil samples were screened on the basis of clear zone formation on agar plates containing emulsified P(64 mol% D-LA-co-3HB). The 16S rRNA analysis of the isolated bacteria resulted in the identification of eight Variovorax, three Acidovorax, and one Burkholderia strains, which are closely related to previously identified natural PHA-degrading bacteria. These bacteria nearly consumed the P(D-LA-co-3HB) emulsion in the liquid culture; however, a small amount of the D-LA fraction remained unconsumed, which should be attributable to the D-LA-clustering structure in the copolymer. Cultivation of the isolated bacteria with the D-LA homo-oligomers revealed that the oligomers with a degree of polymerization (DP) ranging from 10 to 30 were partly consumed by six Variovorax and one Acidovorax strains. In contrast, the oligomers with DP ranging from 20 to 60 were not consumed by the isolated bacteria. These results indicate that D-LA homo-oligomers with DP higher than approximately 20 are hardly degraded by the soil bacteria. Molecular dynamic simulation of the D-LA homo-oligomers indicated that the upper limit of DP is likely to be determined by the conformational structure of the oligomers in water. The information obtained in this study will be useful for the molecular design of biodegradable D-LA-containing polymers.
Rights: © <2020>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松本 謙一郎

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