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Intercellular transport across pit-connections in the filamentous red Griffithsia monilis

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Title: Intercellular transport across pit-connections in the filamentous red Griffithsia monilis
Authors: Kim, Gwang Hoon Browse this author
Nagasato, Chikako Browse this author →KAKEN DB
Kwak, Minseok Browse this author
Lee, Ji Woong Browse this author
Hong, Chan Young Browse this author
Klochkova, Tatyana A. Browse this author
Motomura, Taizo Browse this author →KAKEN DB
Keywords: fluorescent dextran
pit plug
red algae
transfer connection
Issue Date: 15-Mar-2022
Publisher: Korean Soc Phycology
Journal Title: Algae
Volume: 37
Issue: 1
Start Page: 75
End Page: 84
Publisher DOI: 10.4490/algae.2022.37.2.16
Abstract: Intercellular nutrient and signal transduction are essential to sustaining multicellular organisms and maximizing the benefits of multicellularity. It has long been believed that red algal intercellular transport of macromolecules is prevented by the protein-rich pit plug within pit-connections, the only physical connection between cells. Fluorescein isothiocyanate-dextran and recombinant green fluorescence protein (rGFP) of various molecular sizes were injected into vegetative cells of Griffithsia monilis using a micromanipulator, and intercellular transport of the fluorescent probes was examined. Pit-connections were found to provide intercellular transport of tracers at rates comparable to plasmodesmata in other organisms. The time necessary for the transport to an adjacent cell was dependent on the molecular size and the direction of the transport. Fluorescent dextran of 3 kDa was transported to adjacent cells in 1-2 h after injection and migrated to all cells of the filament within 24 h, but fluorescent dextran of 10-20 kDa took 24 h to transfer to neighboring cells. The migration occurred faster towards adjacent reproductive cells and to apical cells than basally. Fluorescent tracers above 40 kDa and rGFP was not transported to neighboring cells, but accumulated near the pit plug. Our results suggest that pit-connections are conduit for macromolecules between neighboring cells and that these size-specific conduits allow intercellular communication between the vegetative cells of red algae.
Type: article
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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