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Intercellular transport across pit-connections in the filamentous red Griffithsia monilis
| Title: | Intercellular transport across pit-connections in the filamentous red Griffithsia monilis |
| Authors: | Kim, Gwang Hoon Browse this author | | Nagasato, Chikako Browse this author →KAKEN DB | | Kwak, Minseok Browse this author | | Lee, Ji Woong Browse this author | | Hong, Chan Young Browse this author | | Klochkova, Tatyana A. Browse this author | | Motomura, Taizo Browse this author →KAKEN DB |
| Keywords: | fluorescent dextran | | microinjection | | pit plug | | red algae | | transfer connection |
| Issue Date: | 15-Mar-2022 |
| Publisher: | Korean Soc Phycology |
| Journal Title: | Algae |
| Volume: | 37 |
| Issue: | 1 |
| Start Page: | 75 |
| End Page: | 84 |
| Publisher DOI: | 10.4490/algae.2022.37.2.16 |
| Abstract: | Intercellular nutrient and signal transduction are essential to sustaining multicellular organisms and maximizing the benefits of multicellularity. It has long been believed that red algal intercellular transport of macromolecules is prevented by the protein-rich pit plug within pit-connections, the only physical connection between cells. Fluorescein isothiocyanate-dextran and recombinant green fluorescence protein (rGFP) of various molecular sizes were injected into vegetative cells of Griffithsia monilis using a micromanipulator, and intercellular transport of the fluorescent probes was examined. Pit-connections were found to provide intercellular transport of tracers at rates comparable to plasmodesmata in other organisms. The time necessary for the transport to an adjacent cell was dependent on the molecular size and the direction of the transport. Fluorescent dextran of 3 kDa was transported to adjacent cells in 1-2 h after injection and migrated to all cells of the filament within 24 h, but fluorescent dextran of 10-20 kDa took 24 h to transfer to neighboring cells. The migration occurred faster towards adjacent reproductive cells and to apical cells than basally. Fluorescent tracers above 40 kDa and rGFP was not transported to neighboring cells, but accumulated near the pit plug. Our results suggest that pit-connections are conduit for macromolecules between neighboring cells and that these size-specific conduits allow intercellular communication between the vegetative cells of red algae. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/85711 |
| Appears in Collections: | 北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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