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Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters

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Title: Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters
Authors: Yamamura, Taiki Browse this author
Narumi, Katsuya Browse this author →KAKEN DB
Ohata, Tsukika Browse this author
Satoh, Hiroshi Browse this author
Mori, Takao Browse this author
Furugen, Ayako Browse this author →KAKEN DB
Kobayashi, Masaki Browse this author →KAKEN DB
Iseki, Ken Browse this author →KAKEN DB
Keywords: Deoxyribonucleoside
Concentrative nucleoside transporter
Issue Date: 18-Jun-2021
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 558
Start Page: 120
End Page: 125
Publisher DOI: 10.1016/j.bbrc.2021.04.075
Abstract: Human concentrative nucleoside transporters (CNTs) are responsible for cellular uptake of ribonucleosides; however, although it is important to better characterize CNT-subtype specificity to understand the systemic disposition of deoxyribonucleosides (dNs) and their analogs, the involvement of CNTs in transporting dNs is not fully understood. In this study, using COS-7 cells that transiently expressed CNT1, CNT2, or CNT3, we investigated if CNTs could transport not only ribonucleosides but also dNs, i.e., 2'-deoxyadenosine (dAdo), 2'-deoxyguanosine (dGuo), and 2'-deoxycytidine (dCyd). The cellular uptake study demonstrated that dAdo and dGuo were taken up by CNT2 but not by CNT1. Although dCyd was taken up by CNT1, no significant uptake was detected in COS-7 cells expressing CNT2. Similarly, these dNs were transported by CNT3. The apparent K-m values of their uptake were as follows: CNT1, K-m = 141 mu M for dCyd; CNT2, K-m = 62.4 mM and 54.9 mu M for dAdo and dGuo, respectively; CNT3, K-m = 14.7 mM and 34.4 mu M for dGuo and dCyd, respectively. These results demonstrate that CNTs contribute not only to ribonucleoside transport but also to the transport of dNs. Moreover, our data indicated that CNT1 and CNT2 selectively transported pyrimidine and purine dNs, respectively, and CNT3 was shown to transport both pyrimidine and purine dNs.
Rights: © <2021>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鳴海 克哉

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