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First detection of SARS-CoV-2 RNA in wastewater in North America : A study in Louisiana, USA
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| Title: | First detection of SARS-CoV-2 RNA in wastewater in North America : A study in Louisiana, USA |
| Authors: | Sherchan, Samendra P. Browse this author | | Shahin, Shalina Browse this author | | Ward, Lauren M. Browse this author | | Tandukar, Sarmila Browse this author | | Aw, Tiong G. Browse this author | | Schmitz, Bradley Browse this author | | Ahmed, Warish Browse this author | | Kitajima, Masaaki Browse this author |
| Keywords: | Wastewater-based epidemiology | | SARS-CoV-2 | | Surveillance | | COVTD-19 | | RT-qPCR | | Wastewater |
| Issue Date: | 15-Nov-2020 |
| Publisher: | Elsevier |
| Journal Title: | Science of the total environment |
| Volume: | 743 |
| Start Page: | 140621 |
| Publisher DOI: | 10.1016/j.scitotenv.2020.140621 |
| Abstract: | We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A). and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SAR.S-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RTqPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater. |
| Rights: | © <2020>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/86171 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 北島 正章
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