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First detection of SARS-CoV-2 RNA in wastewater in North America : A study in Louisiana, USA

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Title: First detection of SARS-CoV-2 RNA in wastewater in North America : A study in Louisiana, USA
Authors: Sherchan, Samendra P. Browse this author
Shahin, Shalina Browse this author
Ward, Lauren M. Browse this author
Tandukar, Sarmila Browse this author
Aw, Tiong G. Browse this author
Schmitz, Bradley Browse this author
Ahmed, Warish Browse this author
Kitajima, Masaaki Browse this author
Keywords: Wastewater-based epidemiology
Issue Date: 15-Nov-2020
Publisher: Elsevier
Journal Title: Science of the total environment
Volume: 743
Start Page: 140621
Publisher DOI: 10.1016/j.scitotenv.2020.140621
Abstract: We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A). and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SAR.S-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RTqPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater.
Rights: © <2020>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 北島 正章

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