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Identification of embryonic RNA granules that act as sites of mRNA translation after changing their physical properties

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Title: Identification of embryonic RNA granules that act as sites of mRNA translation after changing their physical properties
Authors: Sato, Keisuke Browse this author
Sakai, Moeko Browse this author
Ishii, Anna Browse this author
Maehata, Kaori Browse this author
Takada, Yuki Browse this author
Yasuda, Kyota Browse this author →KAKEN DB
Kotani, Tomoya Browse this author →KAKEN DB
Issue Date: 17-Jun-2022
Publisher: Cell Press
Journal Title: iScience
Volume: 25
Issue: 6
Start Page: 104344
Publisher DOI: 10.1016/j.isci.2022.104344
Abstract: Fertilized eggs begin to translate mRNAs at appropriate times and placements to control development, but how the translation is regulated remains unclear. Here, we found that pou5f3 mRNA encoding a transcriptional factor essential for development formed granules in a dormant state in zebrafish oocytes. Although the number of pou5f3 granules remained constant, Pou5f3 protein accumulated after fertilization. Intriguingly, signals of newly synthesized peptides and a ribosomal protein became colocalized with pou5f3 granules after fertilization and, moreover, nascent Pou5f3 was shown to be synthesized in the granules. This functional change was accompanied by changes in the state and internal structure of granules. Dissolution of the granules reduced the rate of protein synthesis. Similarly, nanog and sox19b mRNAs in zebrafish and Pou5f1/Oct4 mRNA in mouse assembled into granules. Our results reveal that subcellular compartments, termed embryonic RNA granules, function as activation sites of translation after changing physical properties for directing vertebrate development.
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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