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Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system

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Title: Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system
Authors: Kitajima, Masaaki Browse this author
Cruz, Mercedes C. Browse this author
Williams, Rohan B. H. Browse this author
Wuertz, Stefan Browse this author
Whittle, Andrew J. Browse this author
Keywords: Water distribution system
Water quality
16S rRNA gene sequencing
Issue Date: 20-Apr-2021
Publisher: Elsevier
Journal Title: Science of the total environment
Volume: 766
Start Page: 142314
Publisher DOI: 10.1016/j.scitotenv.2020.142314
Abstract: Collecting biofilm samples from drinking water distribution systems (DWDSs) is challenging due to limited access to the pipes during regular operations. We report here the analysis of microbial communities in biofilm and water samples collected from sensors installed in a DWDS where monochloramine is used as a residual disinfectant. A total of 52 biofilm samples and 14 bulk water samples were collected from 17 pipe sections representing different water ages. Prokaryotic genome copies (bacterial and archaeal 16S rRNA genes, Mycobacterium spp., ammonia-oxidizing bacteria (AOB), and cyanobacteria) were quantified with droplet digital PCR, which revealed the abundance of these genes in both biofilm and water samples. Prokaryotic 16S rRNA gene sequencing analysis was carried out for a subset of the samples (12 samples from four sites). Mycobacterium and AOB species were dominant in the DWDS sections with low water age and sufficient residual monochloramine, whereas Nitrospira species (nitrite-oxidizing bacteria) dominated in the sections with higher water age and depleted monochloramine level, suggesting the occurrence of nitrification in the studied DWDS. The present study provides novel information on the abundance and identity of prokaryotes in biofilms and water in a full-scale operational DWDS.
Rights: © <2021>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 北島 正章

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