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Small-molecule inhibitor cocktail promotes the proliferation of pre-existing liver progenitor cells
| Title: | Small-molecule inhibitor cocktail promotes the proliferation of pre-existing liver progenitor cells |
| Authors: | Fu, Qingjie Browse this author | | Ohnishi, Shunsuke Browse this author →KAKEN DB | | Suda, Goki Browse this author →KAKEN DB | | Sakamoto, Naoya Browse this author →KAKEN DB |
| Issue Date: | 12-Jul-2022 |
| Publisher: | Cell Press |
| Journal Title: | Stem Cell Reports |
| Volume: | 17 |
| Issue: | 7 |
| Start Page: | 1589 |
| End Page: | 1603 |
| Publisher DOI: | 10.1016/j.stemcr.2022.05.023 |
| Abstract: | A recent study showed that a cocktail of three small molecules, Y-27632, A83-01, and CHIR99021 (YAC), converts mature hepatocytes (MHs) into proliferative bipotent cells that can be induced into MHs and cholangiocytes in rats. However, when we reproduced these experiments, it was found that bipotent cells may be derived from resident liver progenitor cells (LPCs), whose proliferative activity was promoted by YAC. A simple and efficient sorting scheme was also developed in this study to harvest high-purity and high-yield LPCs. The inducible bipotency of purified LPCs was verified; in addition, they were found to spontaneously differentiate into hepatocytes and cholangiocytes due to changes in proliferative status even without induction. Moreover, during the differentiation process, some hepatocytes spontaneously reconverted to LPCs under certain conditions, such as the release of contact inhibition. These findings may improve our understanding of LPCs and provide a cell source for regenerative medicine. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86991 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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