Title: | The detectability and removal efficiency of SARS-CoV-2 in a large-scale septic tank of a COVID-19 quarantine facility in Japan |
Authors: | Iwamoto, Ryo Browse this author |
Yamaguchi, Kiyoshi Browse this author |
Arakawa, Chisato Browse this author |
Ando, Hiroki Browse this author |
Haramoto, Eiji Browse this author |
Setsukinai, Ken-ichi Browse this author |
Katayama, Kotoe Browse this author |
Yamagishi, Takuya Browse this author |
Sorano, Sumire Browse this author |
Murakami, Michio Browse this author |
Kyuwa, Shigeru Browse this author |
Kobayashi, Hiroyuki Browse this author →KAKEN DB |
Okabe, Satoshi Browse this author →KAKEN DB |
Imoto, Seiya Browse this author |
Kitajima, Masaaki Browse this author |
Keywords: | SARS-CoV-2 |
Wastewater-based epidemiology |
Wastewater treatment |
Activated sludge |
Quantification method |
Issue Date: | 25-Nov-2022 |
Publisher: | Elsevier |
Journal Title: | Science of the total environment |
Volume: | 849 |
Start Page: | 157869 |
Publisher DOI: | 10.1016/j.scitotenv.2022.157869 |
Abstract: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known to be present in sewage, and wastewater -based epidemiology has attracted much attention. However, the physical partitioning of SARS-CoV-2 in wastewater and the removal efficiency of treatment systems require further investigation. This study aimed to investigate the de-tectability and physical partitioning of SARS-CoV-2 in wastewater and assess its removal in a large-scale septic tank employing anaerobic, anoxic, and oxic processes in a sequential batch reactor, which was installed in a coronavirus disease 2019 (COVID-19) quarantine facility. The amount of SARS-CoV-2 RNA in wastewater was determined with polyethylene glycol (PEG) precipitation followed by quantitative polymerase chain reaction (qPCR), and the association of SARS-CoV-2 with wastewater solids was evaluated by the effect of filtration prior to PEG precipitation (pre-filtration). The amount of SARS-CoV-2 RNA detected from pre-filtered samples was substantially lower than that of samples without pre-filtration. These results suggest that most SARS-CoV-2 particles in wastewater are associ-ated with the suspended solids excluded by pre-filtration. The removal efficiency of SARS-CoV-2 in the septic tank was evaluated based on the SARS-CoV-2 RNA concentrations in untreated and treated wastewater, which was determined by the detection method optimized in this study. Escherichia coli and pepper mild mottle virus (PMMoV) were also quantified to validate the wastewater treatment system's performance. The mean log10 reduction values of SARS-CoV-2, E. coli, and PMMoV were 2.47 (range, 2.25-2.68), 2.81 (range, 2.45-3.18), and 0.66 (range, 0.61-0.70), respec-tively, demonstrating that SARS-CoV-2 removal by the wastewater treatment system was comparable to or better than the removal of fecal indicators. These results suggest that SARS-CoV-2 can be readily removed by the septic tank. This is the first study to determine the removal efficiency of SARS-CoV-2 in a facility-level sequencing batch activated sludge system. |
Type: | article |
URI: | http://hdl.handle.net/2115/87081 |
Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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