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Peer-reviewed Journal Articles, etc >
The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary
| Title: | The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary |
| Authors: | Tsunemine, Satoru Browse this author | | Nakagawa, Hiromi Browse this author | | Suzuki, Yutaka Browse this author | | Murakami, Yota Browse this author →KAKEN DB |
| Issue Date: | 28-Oct-2022 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic acids research |
| Volume: | 50 |
| Issue: | 19 |
| Start Page: | 10914 |
| End Page: | 10928 |
| Publisher DOI: | 10.1093/nar/gkac827 |
| Abstract: | Centromeres of most eukaryotes consist of two distinct chromatin domains: a kinetochore domain, identified by the histone H3 variant, CENP-A, and a heterochromatic domain. How these two domains are separated is unclear. Here, we show that, in Schizosaccharomyces pombe, mutation of the chromatin remodeler RSC induced CENP-A(Cnp1) misloading at pericentromeric heterochromatin, resulting in the mis-assembly of kinetochore proteins and a defect in chromosome segregation. We find that RSC functions at the kinetochore boundary to prevent CENP-A(Cnp1) from spreading into neighbouring heterochromatin, where deacetylated histones provide an ideal environment for the spread of CENP-A(Cnp1). In addition, we show that RSC decompacts the chromatin structure at this boundary, and propose that this RSC-directed chromatin decompaction prevents mis-propagation of CENP-A(Cnp1) into pericentromeric heterochromatin. Our study provides an insight into how the distribution of distinct chromatin domains is established and maintained. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/87087 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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