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The cell type-specific ER membrane protein UGS148 is not essential in mice
| Title: | The cell type-specific ER membrane protein UGS148 is not essential in mice |
| Authors: | Takahashi, Osamu Browse this author | | Tanahashi, Mayuko Browse this author | | Yokoi, Saori Browse this author | | Kaneko, Mari Browse this author | | Yanaka, Kaori Browse this author | | Nakagawa, Shinichi Browse this author →KAKEN DB | | Maita, Hiroshi Browse this author →KAKEN DB |
| Keywords: | endoplasmic reticulum | | IDR | | mitochondria | | nervous system | | noncoding RNA | | tanycytes |
| Issue Date: | 12-Dec-2021 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Genes to cells |
| Volume: | 27 |
| Issue: | 1 |
| Start Page: | 43 |
| End Page: | 60 |
| Publisher DOI: | 10.1111/gtc.12910 |
| Abstract: | Genomes of higher eukaryotes encode many uncharacterized proteins, and the functions of these proteins cannot be predicted from the primary sequences due to a lack of conserved functional domains. In this study, we focused on a poorly characterized protein UGS148 that is highly expressed in a specialized cell type called tanycytes that line the ventral wall of the third ventricle in the hypothalamus. Immunostaining of UGS148 revealed the fine morphology of tanycytes with highly branched apical ER membranes. Immunoprecipitation revealed that UGS148 associated with mitochondrial ATPase at least in vitro, and ER and mitochondrial signals occasionally overlapped in tanycytes. Mutant mice lacking UGS148 did not exhibit overt phenotypes, suggesting that UGS148 was not essential in mice reared under normal laboratory conditions. We also found that RNA probes that were predicted to uniquely detect UGS148 mRNA cross-reacted with uncharacterized RNAs, highlighting the importance of experimental validation of the specificity of probes during the hybridization-based study of RNA localization. |
| Rights: | This is the peer reviewed version of the following article: https://onlinelibrary.wiley.com/doi/full/10.1111/gtc.12910, which has been published in final form at https://doi.org/10.1111/gtc.12910. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/87675 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 中川 真一
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