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New azodyrecins identified by a genome mining-directed reactivity-based screening
| Title: | New azodyrecins identified by a genome mining-directed reactivity-based screening |
| Authors: | Choirunnisa, Atina Rizkiya Browse this author | | Arima, Kuga Browse this author | | Abe, Yo Browse this author | | Kagaya, Noritaka Browse this author | | Kudo, Kei Browse this author | | Suenaga, Hikaru Browse this author | | Hashimoto, Junko Browse this author | | Fujie, Manabu Browse this author | | Satoh, Noriyuki Browse this author | | Shin-ya, Kazuo Browse this author | | Matsuda, Kenichi Browse this author →KAKEN DB | | Wakimoto, Toshiyuki Browse this author →KAKEN DB |
| Keywords: | biosynthesis | | methyltransferase | | natural azoxides | | reactivity-based screening | | Streptomyces |
| Issue Date: | 10-Aug-2022 |
| Publisher: | Beilstein-Institut |
| Journal Title: | Beilstein journal of organic chemistry |
| Volume: | 18 |
| Start Page: | 1017 |
| End Page: | 1025 |
| Publisher DOI: | 10.3762/bjoc.18.102 |
| Abstract: | Only a few azoxy natural products have been identified despite their intriguing biological activities. Azodyrecins D-G, four new analogs of aliphatic azoxides, were identified from two Streptomyces species by a reactivity-based screening that targets azoxy bonds. A biological activity evaluation demonstrated that the double bond in the alkyl side chain is important for the cytotoxicity of azodyrecins. An in vitro assay elucidated the tailoring step of azodyrecin biosynthesis, which is mediated by the S-adenosylmethionine (SAM)-dependent methyltransferase Ady1. This study paves the way for the targeted isolation of aliphatic azoxy natural products through a genome-mining approach and further investigations of their biosynthetic mechanisms. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/87711 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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