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Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria

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Title: Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria
Authors: Oshiki, Mamoru Browse this author →KAKEN DB
Fukushima, Toshikazu Browse this author
Kawano, Shuichi Browse this author
Nakagawa, Junichi Browse this author
Keywords: recombination amplification reaction (RPA)
endpoint assay
gene quantification
thiocyanate dehydrogenase
Issue Date: 2022
Publisher: Japanese Society of Microbial Ecology, Department of Bioresource Science
Journal Title: Microbes and environments
Volume: 37
Issue: 1
Start Page: ME21073
Publisher DOI: 10.1264/jsme2.ME21073
Abstract: An endpoint recombination amplification reaction (RPA) assay for assessing the abundance of the gene encoding thiocyanate dehydrogenase (TcDH) in Thiohalobacter has been developed. The RPA reaction was performed at 37 degrees C for 30 min, terminated by the addition of sodium dodecyl sulfate (SDS) solution, and the DNA concentration of the RPA product was fluorometrically measured. The abundance of TcDH in 22 activated sludge samples and 7 thiocyanate-degrading enrichment cultures ranged between 2.5x10(3) and 1.5x10(6) copies mu L-1, showing a linear relationship (R-2=0.83) with those measured using a conventional quantitative PCR assay.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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