Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Electron Paramagnetic Resonance Implemented with Multiple Harmonic Detections Successfully Maps Extracellular pH In Vivo
Title: | Electron Paramagnetic Resonance Implemented with Multiple Harmonic Detections Successfully Maps Extracellular pH In Vivo |
Authors: | Nakaoka, Ririko Browse this author | Kato, Kazuhiro Browse this author | Yamamoto, Kumiko Browse this author | Yasui, Hironobu Browse this author →KAKEN DB | Matsumoto, Shingo Browse this author →KAKEN DB | Kirilyuk, Igor A. Browse this author | Khramtsov, Valery V. Browse this author | Inanami, Osamu Browse this author →KAKEN DB | Hirata, Hiroshi Browse this author →KAKEN DB |
Issue Date: | 28-Feb-2023 |
Publisher: | American Chemical Society |
Journal Title: | Analytical chemistry |
Volume: | 95 |
Issue: | 8 |
Start Page: | 3940 |
End Page: | 3950 |
Publisher DOI: | 10.1021/acs.analchem.2c03194 |
Abstract: | Extracellular acidification indicates a metabolic shift in cancer cells and is, along with tissue hypoxia, a hallmark of tumor malignancy. Thus, non-invasive mapping of extracellular pH (pHe) is essential for researchers to understand the tumor microenvironment and to monitor tumor response to metabolism targeting drugs. While electron paramagnetic resonance (EPR) has been successfully used to map pHe in mouse xenograft models, this method is not sensitive enough to map pHe with a moderate amount of exogenous pH-sensitive probes. Here, we show that a modified EPR system achieves twofold higher sensitivity by using the multiple harmonic detection (MHD) method and improves the robustness of pHe mapping in mouse xenograft models. Our results demonstrate that treatment of a mouse xenograft model of human-derived pancreatic ductal adenocarcinoma cells with the carbonic anhydrase IX (CAIX) inhibitor U-104 delays tumor growth with a concurrent tendency toward further extracellular acidification. We anticipate that EPR-based pHe mapping can be expanded to monitor the response of other metabolism-targeting drugs. Furthermore, pHe monitoring can also be used for the development of improved metabolism-targeting cancer treatments. |
Type: | article |
URI: | http://hdl.handle.net/2115/88566 |
Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|