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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
| Title: | Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device |
| Authors: | Maeki, Masatoshi Browse this author | | Okada, Yuto Browse this author | | Uno, Shuya Browse this author | | Niwa, Ayuka Browse this author | | Ishida, Akihiko Browse this author →KAKEN DB | | Tani, Hirofumi Browse this author →KAKEN DB | | Tokeshi, Manabu Browse this author →KAKEN DB |
| Issue Date: | 22-Mar-2022 |
| Publisher: | Journal of visualized experiments |
| Journal Title: | JoVE-journal of visualized experiments |
| Volume: | 181 |
| Start Page: | e62999 |
| Publisher DOI: | 10.3791/62999 |
| Abstract: | The development of functional lipid nanoparticles (LNPs) is one of the major challenges in the field of drug delivery systems (DDS). Recently, LNP-based RNA delivery systems, namely, RNA-loaded LNPs have attracted attention for RNA therapy. In particular, mRNA-loaded LNP vaccines were approved to prevent COVID-19, thereby leading to the paradigm shift toward the development of next-generation nanomedicines. For the LNP-based nanomedicines, the LNP size is a significant factor in controlling the LNP biodistribution and LNP performance. Therefore, a precise LNP size control technique is indispensable for the LNP production process. Here, we report a protocol for size controlled LNP production using a microfluidic device, named iLiNP. siRNA loaded LNPs are also produced using the iLiNP device and evaluated by in vitro experiment. Representative results are shown for the LNP size, including siRNA-loaded LNPs, Z-potential, siRNA encapsulation efficiency, cytotoxicity, and target gene silencing activity. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/88644 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 真栄城 正寿
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