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Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

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Title: Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
Authors: Maeki, Masatoshi Browse this author
Okada, Yuto Browse this author
Uno, Shuya Browse this author
Niwa, Ayuka Browse this author
Ishida, Akihiko Browse this author →KAKEN DB
Tani, Hirofumi Browse this author →KAKEN DB
Tokeshi, Manabu Browse this author →KAKEN DB
Issue Date: 22-Mar-2022
Publisher: Journal of visualized experiments
Journal Title: JoVE-journal of visualized experiments
Volume: 181
Start Page: e62999
Publisher DOI: 10.3791/62999
Abstract: The development of functional lipid nanoparticles (LNPs) is one of the major challenges in the field of drug delivery systems (DDS). Recently, LNP-based RNA delivery systems, namely, RNA-loaded LNPs have attracted attention for RNA therapy. In particular, mRNA-loaded LNP vaccines were approved to prevent COVID-19, thereby leading to the paradigm shift toward the development of next-generation nanomedicines. For the LNP-based nanomedicines, the LNP size is a significant factor in controlling the LNP biodistribution and LNP performance. Therefore, a precise LNP size control technique is indispensable for the LNP production process. Here, we report a protocol for size controlled LNP production using a microfluidic device, named iLiNP. siRNA loaded LNPs are also produced using the iLiNP device and evaluated by in vitro experiment. Representative results are shown for the LNP size, including siRNA-loaded LNPs, Z-potential, siRNA encapsulation efficiency, cytotoxicity, and target gene silencing activity.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 真栄城 正寿

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