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Characterization of embB mutations involved in ethambutol resistance in multi-drug resistant Mycobacterium tuberculosis isolates in Zambia
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| Title: | Characterization of embB mutations involved in ethambutol resistance in multi-drug resistant Mycobacterium tuberculosis isolates in Zambia |
| Authors: | Bwalya, Precious Browse this author | | Solo, Eddie S. Browse this author | | Chizimu, Joseph Y. Browse this author | | Shrestha, Dipti Browse this author | | Mbulo, Grace Browse this author | | Thapa, Jeewan Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | Mycobacterium tuberculosis | | Ethambutol | | Multi-drug resistance | | embB mutations | | Zambia |
| Issue Date: | Mar-2022 |
| Publisher: | Elsevier |
| Journal Title: | Tuberculosis |
| Volume: | 133 |
| Start Page: | 102184 |
| Publisher DOI: | 10.1016/j.tube.2022.102184 |
| Abstract: | Background: Ethambutol (EMB) is an important anti-tuberculosis drug used in the management of multi-drug resistant tuberculosis (MDR-TB). Mutations in embB are the major mechanism of resistance. This study investigated embB mutations among MDR-TB isolates and analyzed their correlations with phenotypic drug susceptibility testing (DST) in Zambia. Method: A total of 132 MDR-TB isolates were collected from January 2014 to April 2017 and characterized using MGIT 960 systems, embB sequencing, and spoligotyping. Results: Out of 61 phenotypically EMB resistant isolates, 53 had mutations in embB. Among the 71 EMB susceptible isolates, 47 had embB mutations. Sensitivity of embB mutations was 86.9% while specificity was 33.8%. CAS1_Kili (SIT21) had high odds of having embB mutations, particularly, G918A (Met306eIl) (Odds ratio 16.7, p < 0.0001). Conclusion: Molecular EMB resistance testing by DNA sequencing can improve detection of EMB resistance among MDR-TB patients in Zambia. Additionally, CAS1_Kili was associated with embB amino acid substitution Met306Ile suggesting transmission. A detailed investigation to track and determine transmission hotspot area for MDR-TB could help optimize control strategies. |
| Rights: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/88701 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 定彦
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