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Biosynthetic Gene Cluster of Linaridin Peptides Contains Epimerase Gene

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/89005

Title: Biosynthetic Gene Cluster of Linaridin Peptides Contains Epimerase Gene
Authors: Xiao, Wanlu Browse this author
Satoh, Yasuharu Browse this author →KAKEN DB
Ogasawara, Yasushi Browse this author →KAKEN DB
Dairi, Tohru Browse this author →KAKEN DB
Keywords: biosynthesis
D-amino acids
epimerases
linaridin
peptides
Issue Date: 20-Jun-2022
Publisher: Wiley-Blackwell
Journal Title: Chembiochem
Volume: 23
Issue: 12
Start Page: e202100705
Publisher DOI: 10.1002/cbic.202100705
Abstract: Salinipeptins belong to the type-A linaridin class of ribosomally synthesized and post-translationally modified peptides (RiPPs) comprising 22 amino acid residues with multiple D-amino acids. Although chirality of other type-A linaridins, such as grisemycin and cypemycin, has not been reported, the biosynthetic gene clusters of type-A linaridins have identical gene organization. Here, we report heterologous expression of grisemycin biosynthetic gene cluster (grm) and show that grisemycin contains multiple D-amino acids, similar to salinipeptins. The heterologous expression experiments also confirm the involvement of a novel peptide epimerase in grisemycin biosynthesis. Gene-deletion experiments indicate that grmL, a single gene with unknown function, is indispensable for grisemycin production. We also show that the presence of D-amino acids is likely a common feature of linaridin natural products by analyzing two other type-A linaridin clusters.
Rights: This is the peer reviewed version of the following article: W. Xiao, Y. Satoh, Y. Ogasawara, T. Dairi, ChemBioChem 2022, 23, e202100705, which has been published in final form at https://doi.org/10.1002/cbic.202100705. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Type: article (author version)
URI: http://hdl.handle.net/2115/89005
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小笠原 泰志

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