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Hokkaido University Collection of Scholarly and Academic Papers >
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Screening Antibiotic-Resistant Escherichia coli in Wastewater and River Water Using a Novel Simple Phenotypic Antibiotic-Susceptibility Testing Method
| Title: | Screening Antibiotic-Resistant Escherichia coli in Wastewater and River Water Using a Novel Simple Phenotypic Antibiotic-Susceptibility Testing Method |
| Authors: | Satoh, Hisashi Browse this author →KAKEN DB | | Nagahashi, Natsumi Browse this author | | Kikuchi, Kai Browse this author | | Hirano, Reiko Browse this author |
| Keywords: | β-d-glucuronidase | | microplate reader | | culture-based method | | logarithmic (log) growth phase | | lethal concentration | | fate of antibiotic-resistant Escherichia coli |
| Issue Date: | 12-Aug-2022 |
| Publisher: | American Chemical Society(ACS) |
| Journal Title: | ACS ES&T Water |
| Volume: | 2 |
| Issue: | 8 |
| Start Page: | 1301 |
| End Page: | 1308 |
| Publisher DOI: | 10.1021/acsestwater.1c00359 |
| Abstract: | Recently, wastewater treatment plants have been identified as potential reservoirs of antibiotic-resistant bacteria. Hence, it is important to monitor antibiotic-resistant bacteria in wastewater treatment plants. Currently available methods are laborious, time-consuming, and costly. In this study, a novel simple phenotypic antibiotic-susceptibility testing method for Escherichia coli was developed. A growth curve for E. coli in the presence of an antibiotic was established by monitoring the fluorescence intensity of a fluorogenic substrate specific to E. coli using a microplate reader. The antibiotic-resistant E. coli to total E. coli ratios for wastewater and river water samples were determined using fluorescence intensity measurements. The lethal concentrations of antibiotics to E. coli could be roughly estimated using the method. The lethal concentrations were dependent on the antibiotic type rather than differences between the wastewater treatment plants and were higher for samples from primary clarifiers than secondary clarifiers in wastewater treatment plants. The lethal concentrations for river water decreased as the distance from the wastewater treatment plant discharge outlet increased. The novel simple phenotypic antibiotic-susceptibility testing method allows antibiotic-resistant E. coli concentrations in wastewater and river water to be determined rapidly with a high throughput and will allow effective and timely decisions to be made to control antibiotic-resistant E. coli. |
| Rights: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS ES&T Water, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/articlesonrequest/AOR-3ECWFWRNIKH9EPJ4ZUKR. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90044 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐藤 久
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