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Development of an Absorbance Detection Module Using a Deep UV Light-Emitting Diode for a Portable Miniaturized Liquid Chromatograph

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Title: Development of an Absorbance Detection Module Using a Deep UV Light-Emitting Diode for a Portable Miniaturized Liquid Chromatograph
Other Titles: ポータブル小型液体クロマトグラフのための深紫外発光ダイオードベースの吸光度検出モジュールの開発
Authors: Koyama, Kaito Browse this author
Nishinura, Takuma Browse this author
Ishida, Akihiko Browse this author →KAKEN DB
Hibino, Mitsue Browse this author
Maeki, Masatoshi Browse this author
Tani, Hirofumi Browse this author →KAKEN DB
Tokeshi, Manabu Browse this author →KAKEN DB
Keywords: portable liquid chromatograph
absorbance detector
flow cell
Issue Date: 5-Mar-2023
Publisher: 日本分析化学会(Japan Society for Analytical Chemistry)
Journal Title: 分析化学(Bunseki Kagaku)
Volume: 72
Issue: 3
Start Page: 125
End Page: 131
Publisher DOI: 10.2116/bunsekikagaku.72.125
Abstract: In recent years, there has been increasing demand for miniaturized analytical instruments that allow immediate on-site analysis. We have been developing a portable HPLC system consisting of a column-integrated chip and a compact, low-power electroosmotic flow pump. This study aims to extend the versatility of the portable HPLC system with a further reduction of the overall system size, and thus we constructed a UV absorbance detection module that can be mounted to the column-integrated chip. For this detection module, a UV light-emitting diode (peak wavelength = 255 nm) and photodiode were used as a light source and photodetector, and a system to control their operation and process the signals was also constructed. The developed detection module exhibited excellent performances as an HPLC detector.
近年,サンプリングした現場で即時に分析が可能な小型分析装置の需要が高まっている.著者らは,小型・低電力の電気浸透流ポンプとカラム内蔵チップからなる小型HPLCシステムの開発に取り組んできた.本研究では,小型HPLCシステム全体のさらなる小型化を目指しながら検出における汎用性を拡張するため,カラム内蔵チップに適用可能で,汎用性が高い紫外吸光度検出モジュールを構築した.そのために光源及び受光器に深紫外発光ダイオード(ピーク発光波長255 nm)及びフォトダイオードを用いるとともに,これらの動作を制御し,信号を処理するシステムも構築した.さらに,検出モジュールが市販分光光度計並みの感度を持ち,良好にクロマトグラム測定ができることを実証した.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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