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Converting cytochrome c into a DyP-like metalloenzyme
| Title: | Converting cytochrome c into a DyP-like metalloenzyme |
| Authors: | Omura, Issei Browse this author | | Ishimori, Koichiro Browse this author →KAKEN DB | | Uchida, Takeshi Browse this author →KAKEN DB |
| Issue Date: | 7-Sep-2022 |
| Publisher: | Royal Society of Chemistry |
| Journal Title: | Dalton Transactions |
| Volume: | 51 |
| Issue: | 33 |
| Start Page: | 12641 |
| End Page: | 12649 |
| Publisher DOI: | 10.1039/D2DT02137D |
| Abstract: | Dye-decolorizing peroxidase (DyP), which can degrade anthraquinone dyes using H2O2, is an attractive prospect for potential biotechnological applications for environmental purification. We previously designed an artificial DyP with an optimal pH for reactive blue 19 (RB19) degradation shifting from pH 4.5 to 6.5. We then attempted to degrade RB19 using Escherichia coli expressing this mutant, but RB19 was degraded equally compared with bacteria expressing wild-type (WT) DyP because most DyP was expressed in a heme-free form. In this study, we attempted to design an artificial peroxidase based on cytochrome c (cyt c), whose heme is covalently bound to the protein. We found that cyt c can degrade RB19, but its ability at pH 7.0 was ~60% of that of DyP from Vibrio cholerae at pH 4.5. To enhance this activity we constructed several mutants using three approaches. Initially, to improve reactivity with H2O2, Met80 was replaced with a noncoordinating residue, Ala or Val, but catalytic efficiency (kcat/Km) was increased by only ~1.5-fold. To enhance the substrate binding affinity we introduced an additional Trp by replacing Pro76 (P76W). The catalytic efficiency of this mutant was ~3-fold greater than that of WT cyt c. Finally, to form a hydrogen bond to axial histidine Gly29 was replaced with Asp (G29D). This mutant exhibited an ~80-fold greater dye-decolorizing activity. Escherichia coli expressing the G29D mutant was unable to degrade RB19 in solution due to degradation of heme itself, but this study provides new insights into the design of artificial DyPs. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90345 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 内田 毅
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