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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Application of chemostat culture to nutrient uptake rate measurements by the macroalgae Saccharina japonica var. religiosa (Phaeophyceae) and Ulva australlis (Ulvophyceae)
| Title: | Application of chemostat culture to nutrient uptake rate measurements by the macroalgae Saccharina japonica var. religiosa (Phaeophyceae) and Ulva australlis (Ulvophyceae) |
| Authors: | Okazaki, Ryosuke Browse this author | | Teramoto, Narumi Browse this author | | Carlson, Andrew K. Browse this author | | Nakanishi, Kiyoko Browse this author | | Kudo, Isao Browse this author →KAKEN DB |
| Keywords: | batch culture | | chemostat culture | | macroalgae | | Michaelis-Menten curve | | nutrient uptake kinetics |
| Issue Date: | 8-Sep-2023 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Phycological research |
| Publisher DOI: | 10.1111/pre.12483 |
| Abstract: | In this study, we applied a chemostat culture method, for the first time, to measure the nutrient uptake rate of macroalgae. We examined two methods of measuring the nutrient uptake rate of two macroalgae, Saccharina japonica var. religiosa and Ulva australis, by comparing nutrient uptake kinetics between the chemostat culture and batch culture. In the chemostat culture, the nutrient concentration was kept constant by monitoring the change in nutrient concentration using an Auto Analyzer in real time and adding nutrients to compensate for the macroalgae's nutrient consumption. The nutrient uptake in the chemostat culture could be best fitted to the Michaelis-Menten saturation kinetics. In the batch culture, the nutrient concentration decreased with time, either constantly or exponentially due to a rapid uptake of nutrients by the macroalgae. The nutrient uptake rate in the batch culture generally showed a scattered relationship with nutrient concentration, with a weak fitting to the Michaelis-Menten saturation kinetics. This discrepancy seemed to be partly because the change in nutrient concentration was large between the sampling intervals in the batch culture. Determining an appropriate sampling interval for detectable concentration change is difficult unless the nutrient concentration is measured in real time. Therefore, the application of the chemostat culture method to the measurement of the uptake rate by macroalgae could greatly improve our understanding of nutrient uptake kinetics. |
| Rights: | This is the peer reviewed version of the following article: Okazaki, R., Teramoto, N., Carlson, A.K., Nakanishi, K. and Kudo, I. (2022), Application of chemostat culture to nutrient uptake rate measurements by the macroalgae Saccharina japonica var. religiosa (Phaeophyceae) and Ulva australis (Ulvophyceae). Phycological Res., 70: 142-150, which has been published in final form at https://doi.org/10.1111/pre.12483. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90347 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 工藤 勲
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