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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
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Nicotinic acetylcholine receptors in a songbird brain
| Title: | Nicotinic acetylcholine receptors in a songbird brain |
| Authors: | Asogwa, Norman Chinweike Browse this author | | Toji, Noriyuki Browse this author →KAKEN DB | | He, Ziwei Browse this author | | Shao, Chengru Browse this author | | Shibata, Yukino Browse this author | | Tatsumoto, Shoji Browse this author | | Ishikawa, Hiroe Browse this author | | Go, Yasuhiro Browse this author | | Wada, Kazuhiro Browse this author →KAKEN DB |
| Keywords: | acetylcholine | | nicotinic receptors | | sensorimotor learning | | vocal learning | | zebra finch |
| Issue Date: | Aug-2022 |
| Publisher: | John Wiley & Sons |
| Journal Title: | The journal of comparative neurology |
| Volume: | 530 |
| Issue: | 11 |
| Start Page: | 1966 |
| End Page: | 1991 |
| Publisher DOI: | 10.1002/cne.25314 |
| Abstract: | Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that mediate fast synaptic transmission and cell signaling, which contribute to learning, memory, and the execution of motor skills. Birdsong is a complex learned motor skill in songbirds. Although the existence of 15 nAChR subunits has been predicted in the avian genome, their expression patterns and potential contributions to song learning and production have not been comprehensively investigated. Here, we cloned all the 15 nAChR subunits (ChrnA1-10, B2-4, D, and G) from the zebra finch brain and investigated the mRNA expression patterns in the neural pathways responsible for the learning and production of birdsong during a critical period of song learning. Although there were no detectable hybridization signals for ChrnA1, A6, A9, and A10, the other 11 nAChR subunits were uniquely expressed in one or more major subdivisions in the song nuclei of the songbird brain. Of these 11 subunits, ChrnA3-5, A7, and B2 were differentially regulated in the song nuclei compared with the surrounding anatomically related regions. ChrnA5 was upregulated during the critical period of song learning in the lateral magnocellular nucleus of the anterior nidopallium. Furthermore, single-cell RNA sequencing revealed ChrnA7 and B2 to be the major subunits expressed in neurons of the vocal motor nuclei HVC and robust nucleus of the arcopallium, indicating the potential existence of ChrnA7-homomeric and ChrnB2-heteromeric nAChRs in limited cell populations. These results suggest that relatively limited types of nAChR subunits provide functional contributions to song learning and production in songbirds. |
| Rights: | This is the peer reviewed version of the following article: Asogwa, N. C., Toji, N., He, Z., Shao, C., Shibata, Y., Tatsumoto, S., Ishikawa, H., Go, Y., & Wada, K. (2022). Nicotinic acetylcholine receptors in a songbird brain. J Comp Neurol, 530, 1966– 1991. , which has been published in final form at https://doi.org/10.1002/cne.25314. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90351 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 和多 和宏
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