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Dopamine regulates astrocytic IL-6 expression and process formation via dopamine receptors and adrenoceptors
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| Title: | Dopamine regulates astrocytic IL-6 expression and process formation via dopamine receptors and adrenoceptors |
| Authors: | Morimoto, Kohei Browse this author | | Ouchi, Mai Browse this author | | Kitano, Taisuke Browse this author | | Eguchi, Ryota Browse this author | | Otsuguro, Ken-ichi Browse this author →KAKEN DB |
| Keywords: | Astrocyte | | Dopamine | | Adrenoceptor | | IL-6 | | CREB | | Morphology |
| Issue Date: | 5-Aug-2022 |
| Publisher: | Elsevier |
| Journal Title: | European journal of pharmacology |
| Volume: | 928 |
| Start Page: | 175110 |
| Publisher DOI: | 10.1016/j.ejphar.2022.175110 |
| Abstract: | Dopamine levels in the central nervous system change under pathological conditions such as Parkinson's disease, Huntington's disease, and addiction. Under those pathological conditions, astrocytes become reactive astrocytes characterized by morphological changes and the release of inflammatory cytokines involved in pathogenesis. However, it remains unclear whether dopamine regulates astrocytic morphology and functions. Elucidating these issues will help us to understand the pathogenesis of neurodegenerative diseases caused by abnormal dopamine signaling. In this study, we investigated the effects of dopamine on IL-6 expression and process formation in rat primary cultured astrocytes and acute hippocampal slices. Dopamine increased IL-6 expression in a concentration-dependent manner, and this was accompanied by CREB phosphorylation. The effects of a low dopamine concentration (1 mu M) were inhibited by a D1-like receptor antagonist, whereas the effects of a high dopamine concentration (100 mu M) were inhibited by a beta-antagonist and enhanced by a D2-like receptor antagonist. Furthermore, dopamine (100 mu M) promoted process formation, which was inhibited by a beta-antag-onist and enhanced by both an alpha-antagonist and a D2-like receptor antagonist. In acute hippocampal slices, both a D1-like receptor agonist and beta-agonist changed astrocytic morphology. Together, these results indicate that dopamine promotes IL-6 expression and process formation via D1-like receptors and beta-adrenoceptors. Further-more, bidirectional regulation exists; namely, the effects of D1-like receptors and beta-adrenoceptors were nega-tively regulated by D2-like receptors and alpha(2)-adrenoceptors. |
| Rights: | © <2022>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90384 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 乙黒 兼一
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