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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants
| Title: | Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants |
| Authors: | Kawakubo, Ayaka Browse this author | | Gallois, Jean-Luc Browse this author | | Nakahara, Kenji S. Browse this author →KAKEN DB |
| Keywords: | iLOV | | Cucumber mosaic virus | | In vivo monitoring viral spread |
| Issue Date: | 12-Oct-2022 |
| Publisher: | Springer |
| Journal Title: | Journal of general plant pathology |
| Volume: | 89 |
| Start Page: | 47 |
| End Page: | 52 |
| Publisher DOI: | 10.1007/s10327-022-01100-6 |
| Abstract: | Tracking virus infection in plants is useful to assess plant susceptibility or resistance. The recombinant cucumber mosaic virus (CMV) expressing a 2b-fused green fluorescent protein (GFP, 25 kDa) gradually loses (within weeks) the GFP gene in plants. Here, we constructed CMV expressing a flavin-based small fluorescent protein (iLOV, 10 kDa). CMV-iLOV still retains iLOV after 28 dpi in upper leaves of inoculated Nicotiana benthamiana and allows long-term monitoring of its distribution in plants. Using CMV-iLOV, we showed that pot1, a recessive allele for resistance to potyviruses, does not confer CMV resistance in tomato. |
| Rights: | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10327-022-01100-6 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90544 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 中原 健二
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