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Species-specific formation of paraspeckles in intestinal epithelium revealed by characterization of NEAT1 in naked mole-rat
| Title: | Species-specific formation of paraspeckles in intestinal epithelium revealed by characterization of NEAT1 in naked mole-rat |
| Authors: | Yamada, Akihiro Browse this author | | Toya, Hikaru Browse this author | | Tanahashi, Mayuko Browse this author | | Kurihara, Misuzu Browse this author | | Mito, Mari Browse this author | | Iwasaki, Shintaro Browse this author | | Kurosaka, Satoshi Browse this author | | Takumi, Toru Browse this author | | Fox, Archa Browse this author | | Kawamura, Yoshimi Browse this author →KAKEN DB | | Miura, Kyoko Browse this author | | Nakagawa, Shinichi Browse this author →KAKEN DB |
| Keywords: | FUS | | NEAT1 | | naked mole-rat | | paraspeckles |
| Issue Date: | Aug-2022 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Journal Title: | RNA |
| Volume: | 28 |
| Issue: | 8 |
| Start Page: | 1128 |
| End Page: | 1143 |
| Publisher DOI: | 10.1261/rna.079135.122 |
| Abstract: | Paraspeckles are mammalian-specific nuclear bodies built on the long noncoding RNA NEAT1_2. The molecular mechanisms of paraspeckle formation have been mainly studied using human or mouse cells, and it is not known if the same molecular components are involved in the formation of paraspeckles in other mammalian species. We thus investigated the expression pattern of NEAT1_2 in naked mole-rats (nNEAT1_2), which exhibit extreme longevity and lower susceptibility to cancer. In the intestine, nNEAT1_2 is widely expressed along the entire intestinal epithelium, which is different from the expression of mNeat1_2 that is restricted to the cells of the distal tip in mice. Notably, the expression of FUS, a FET family RNA binding protein, essential for the formation of paraspeckles both in humans and mice, was absent in the distal part of the intestinal epithelium in naked mole-rats. Instead, mRNAs of other FET family proteins EWSR1 and TAF15 were expressed in the distal region. Exogenous expression of these proteins in Fus-deficient murine embryonic fibroblast cells rescued the formation of paraspeckles. These observations suggest that nNEAT1_2 recruits a different set of RNA binding proteins in a cell type-specific manner during the formation of paraspeckles in different organisms. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/90649 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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