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Glucose and ecdysteroid increase apyrene sperm production in in vitro cultivation of spermatocysts of Bombyx mori
| Title: | Glucose and ecdysteroid increase apyrene sperm production in in vitro cultivation of spermatocysts of Bombyx mori |
| Authors: | Kawamura, N. Browse this author | | Sahara, K.2 Browse this author →KAKEN DB | | Fugo, H. Browse this author |
| Authors(alt): | 佐原, 健2 |
| Keywords: | Silkworm | | In vitro culture | | Eupyrene and apyrene sperm | | 20-Hydroxyecdysone | | Glucose |
| Issue Date: | 8-Feb-2003 |
| Publisher: | Elsevier Science Ltd. |
| Journal Title: | Journal of Insect Physiology |
| Volume: | 49 |
| Issue: | 1 |
| Start Page: | 25 |
| End Page: | 30 |
| Publisher DOI: | 10.1016/S0022-1910(02)00223-8 |
| Abstract: | Two types of sperm, nucleate eupyrene and anucleate apyrene, occur in the silkworm as in other lepidopteran species. Hormones and other substances have been assumed to play important roles in sperm dimorphism. We established an in vitro cultivation system for silkworm spermatocytes, and found that apyrene sperm are not produced when spermatocytes from larval testes are cultivated, though eupyrene spermatocytes develop normally into mature sperm. Based on the fact that ecdysteroid titers increase rapidly and peak 1 day after spinning, and that the amount of glycogen reaches its peak 1 day before the spinning stage, we studied the effects of adding glucose and/or 20-hydroxyecdysone to the culture medium. The experiments disclosed a significant additive effect of both substances on apyrene sperm production. |
| Relation: | http://www.sciencedirect.com/science/journal/00221910 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/908 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐原 健
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