|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis
| Title: | Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis |
| Authors: | Onishi, Katsuya Browse this author | | Ishihara, Seiichiro Browse this author →KAKEN DB | | Takahashi, Masayuki Browse this author →KAKEN DB | | Sakai, Akihiro Browse this author | | Enomoto, Atsushi Browse this author →KAKEN DB | | Suzuki, Kentaro Browse this author | | Haga, Hisashi Browse this author →KAKEN DB |
| Keywords: | apoptosis | | MafB | | mechanotransduction | | nuclear myosin regulatory light chain (MRLC) | | substrate stiffness | | ZIPK | | DAPK3 |
| Issue Date: | 13-Mar-2023 |
| Publisher: | John Wiley & Sons |
| Journal Title: | FEBS Letters |
| Volume: | 597 |
| Issue: | 5 |
| Start Page: | 643 |
| End Page: | 656 |
| Publisher DOI: | 10.1002/1873-3468.14592 |
| Abstract: | Stiffness of the extracellular matrix regulates various biological responses, but the response mechanisms are poorly understood. Here, we found that the nuclear diphosphorylated myosin regulatory light chain (2P-MRLC) is a critical mechanomediator that suppresses apoptosis in response to substrate stiffness. Stiff substrates promoted the nuclear localization of 2P-MRLC. Zipper-interacting protein kinase [ZIPK; also known as death-associated protein kinase 3 (DAPK3)], a kinase for MRLC, was localized in the nucleus in response to stiff substrates and promoted the nuclear localization of 2P-MRLC. Moreover, actin fiber formation induced by substrate stiffness promoted the nuclear localization of 2P-MRLC via ZIPK. 2P-MRLC in response to substrate stiffness suppressed the expression of MAF bZIP transcription factor B (MafB) and repressed apoptosis. These findings reveal a newly identified role of MRLC in mechanotransduction. |
| Rights: | This is the peer reviewed version of the following article: Onishi, K., Ishihara, S., Takahashi, M., Sakai, A., Enomoto, A., Suzuki, K. and Haga, H. (2023), Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis. FEBS Lett, 597: 643-656., which has been published in final form at Link to final article using the https://doi.org/10.1002/1873-3468.14592. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| Publisher URI: | https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/91296 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 芳賀 永
|
