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Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue

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タイトル: Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
著者: Matsuda, Chikayoshi 著作を一覧する
Takagi, Mutsumi 著作を一覧する
Hattori, Takako 著作を一覧する
Wakitani, Shigeyuki 著作を一覧する
Yoshida, Toshiomi 著作を一覧する
キーワード: Chondrocyte
Differentiation
Mesenchymal stem cell
Three-dimension
発行日: 2005年 1月
出版者: Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
誌名: Cytotechnology
巻: 47
号: 1-3
開始ページ: 11
終了ページ: 17
出版社 DOI: 10.1007/s10616-005-3751-x
抄録: A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-l-lactate-glycolic acid copolymer (PLGA) mesh and cultivated for 4 week together with growth factors. The degree of cellular differentiation was estimated by quantitative RT-PCR of aggrecan and type II collagen mRNAs and by staining with Safranin O. The 3D culture showed a higher degree of differentiation even without growth factors than the conventional pellet culture with growth factors, namely, dexamethasone and transforming growth factor (TGF)-β 3. The 3D culture for 2 week with the combined addition of dexamethasone, TGF-β 3, and insulin-like growth factor (IGF)-I reached a 30% expression of aggrecan mRNA compared with that in primary human chondrocytes, while the aggrecan mRNA expression in the conventional pellet culture was less than 2%. The sequential two-step differentiation cultivation, during which the cells were cultivated in 3D for 1 week after the conventional two-dimensional (2D) culture for 1 week, could markedly accelerate the expression of aggrecan mRNA compared with the 3D cultivation for 2 week.
Rights: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/922
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高木 睦

 

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