|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
| Title: | Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue |
| Authors: | Matsuda, Chikayoshi Browse this author | | Takagi, Mutsumi2 Browse this author →KAKEN DB | | Hattori, Takako Browse this author | | Wakitani, Shigeyuki Browse this author | | Yoshida, Toshiomi Browse this author |
| Authors(alt): | 高木, 睦2 |
| Keywords: | Chondrocyte | | Differentiation | | Mesenchymal stem cell | | Three-dimension |
| Issue Date: | Jan-2005 |
| Publisher: | Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V. |
| Journal Title: | Cytotechnology |
| Volume: | 47 |
| Issue: | 1-3 |
| Start Page: | 11 |
| End Page: | 17 |
| Publisher DOI: | 10.1007/s10616-005-3751-x |
| Abstract: | A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-l-lactate-glycolic acid copolymer (PLGA) mesh and cultivated for 4 week together with growth factors. The degree of cellular differentiation was estimated by quantitative RT-PCR of aggrecan and type II collagen mRNAs and by staining with Safranin O. The 3D culture showed a higher degree of differentiation even without growth factors than the conventional pellet culture with growth factors, namely, dexamethasone and transforming growth factor (TGF)-β 3. The 3D culture for 2 week with the combined addition of dexamethasone, TGF-β 3, and insulin-like growth factor (IGF)-I reached a 30% expression of aggrecan mRNA compared with that in primary human chondrocytes, while the aggrecan mRNA expression in the conventional pellet culture was less than 2%. The sequential two-step differentiation cultivation, during which the cells were cultivated in 3D for 1 week after the conventional two-dimensional (2D) culture for 1 week, could markedly accelerate the expression of aggrecan mRNA compared with the 3D cultivation for 2 week. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/922 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 高木 睦
|
