| Title: | Monocarboxylate Transporters 1 and 2 Are Responsible for L-Lactate Uptake in Differentiated Human Neuroblastoma SH-SY5Y Cells |
| Authors: | Sakuma, Tomoya Browse this author |
| Mukai, Yuto Browse this author |
| Yamaguchi, Atsushi Browse this author |
| Suganuma, Yudai Browse this author |
| Okamoto, Keisuke Browse this author |
| Furugen, Ayako Browse this author →KAKEN DB |
| Narumi, Katsuya Browse this author →KAKEN DB |
| Ishikawa, Shuhei Browse this author →KAKEN DB |
| Saito, Yoshitaka Browse this author →KAKEN DB |
| Kobayashi, Masaki Browse this author →KAKEN DB |
| Keywords: | astrocytes-neuron lactate shuttle |
| monocarboxylate transporter |
| SH-SY5Y cell |
| lactate |
| Issue Date: | 4-Apr-2024 |
| Publisher: | The Pharmaceutical Society of Japan |
| Journal Title: | Biological & pharmaceutical bulletin |
| Volume: | 47 |
| Issue: | 4 |
| Start Page: | 764 |
| End Page: | 770 |
| Publisher DOI: | 10.1248/bpb.b23-00860 |
| Abstract: | L-Lactate transport via monocarboxylate transporters (MCTs) in the central nervous system, represented by the astrocyte-neuron lactate shuttle (ANLS), is crucial for the maintenance of brain functions, including memory formation. Previously, we have reported that MCT1 contributes to L-lactate transport in normal human astrocytes. Therefore, in this study, we aimed to identify transporters that contribute to L-lactate transport in human neurons. SH-SY5Y cells, which are used as a model for human neurons, were differentiated using all-trans-retinoic acid. L-Lactate uptake was measured using radiolabeled L-lactate, and the expression of MCT proteins was confirmed Western blotting. L-Lactate transport was pH-dependent and saturated at high concentrations. Kinetic analysis suggested that L-lactate uptake was biphasic. Furthermore, MCT1, 2 selective inhibitors inhibited L-lactate transport. In addition, the expression of MCT1 and 2 proteins, but not MCT4, was confirmed. In this study, we demonstrated that MCT1 and 2 are major contributors to L-lactate transport in differentiated human neuroblastoma SH-SY5Y cells from the viewpoint of kinetic analysis. These results lead to a better understanding of ANLS in humans, and further exploration of the factors that can promote MCT1 and 2 functions is required. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/92588 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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