| Title: | Elucidation of the biodegradation pathways of bis(2-hydroxyethyl) terephthalate and dimethyl terephthalate under anaerobic conditions revealed by enrichment culture and microbiome analysis |
| Authors: | Kuroda, Kyohei Browse this author |
| Narihiro, Takashi Browse this author |
| Nakaya, Yuki Browse this author |
| Noguchi, Taro Q. P. Browse this author |
| Maeda, Ryota Browse this author |
| Nobu, Masaru K. Browse this author |
| Ohnishi, Yuki Browse this author →KAKEN DB |
| Kumaki, Yasuhiro Browse this author |
| Aizawa, Tomoyasu Browse this author →KAKEN DB |
| Satoh, Hisashi Browse this author →KAKEN DB |
| Keywords: | Polyethyleneterephthalate(PET) |
| Bis(2-hydroxyethyl)terephthalate(BHET) |
| Dimethylterephthalate(DMT) |
| Spirochaeota |
| Esterase |
| Anaerobicbiodegradation |
| Issue Date: | 15-Dec-2022 |
| Publisher: | Elsevier |
| Journal Title: | Chemical engineering journal |
| Volume: | 450 |
| Start Page: | 137916 |
| Publisher DOI: | 10.1016/j.cej.2022.137916 |
| Abstract: | With the globally rising usage of plastics, including polyethylene terephthalate (PET), the environmental risk that disposal of waste plastics to landfills and discharge of microplastics to the marine environment pose have also increased. For example, observation of animal ingestion of fragmented waste plastics (micro-and nano-plastics) has driven awareness for the need of proper environmental risk assessment. In evaluating the biodegradability of PET-derived byproducts and their precursors, most work has focused on hydrolytic enzymes and aerobic or-ganisms that possess such genes, but only few reports on biodegradation in the absence of oxygen (i.e., anaerobic) are available. Here, to elucidate the fate of PET-derived materials under anaerobic environments, a sludge -derived microbial community was cultured with bis(2-hydroxyethyl) terephthalate (BHET) as a model sub-strate for byproducts of PET degradation and dimethyl terephthalate (DMT) as a potential environmental pollutant discharged from the PET manufacturing process. Metagenome-and metabolome-informed microbiome analyses identified anaerobic BHET and DMT degradation pathways, uncultured organisms affiliated with Spi-rochaetota and Negativicutes predominant in the BHET-fed cultures, and Methanomethylovorans and Trepone-ma_G predominant in the DMT-fed cultures. Metagenomic analyses newly identified three BHET-degrading and two DMT-degrading enzymes from the genomes of Spirochaeota. In addition, the Negativicutes in the BHET enrichment cultures possessed genes for acetogenically metabolizing EG and/or ethanol. Overall, this study successfully established anaerobic BHET-and DMT-degrading microbial consortia and newly proposed these degradation mechanisms under anaerobic conditions. This study indicated that the cultivation, microbiome, and metabolome analyses can be powerful tools for elucidating consortia capable of degrading plastics-associated waste compounds and the relevant metabolic mechanisms. |
| Rights: | © <2022>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/92698 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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