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Rapid accumulation of polyphosphate in extraradical hyphae of an arbuscular mycorrhizal fungus as revealed by histochemistry and a polyphosphate kinase/luciferase system
| Title: | Rapid accumulation of polyphosphate in extraradical hyphae of an arbuscular mycorrhizal fungus as revealed by histochemistry and a polyphosphate kinase/luciferase system |
| Authors: | Ezawa, Tatsuhiro1 Browse this author →KAKEN DB | | Cavagnaro, Timothy R. Browse this author | | Smith, Sally E. Browse this author | | Smith, F. Andrew Browse this author | | Ohtomo, Ryo Browse this author |
| Authors(alt): | 江澤, 辰広1 |
| Keywords: | arbuscular mycorrhizal fungi | | luciferase | | metachromasy | | polyphosphate | | polyphosphate kinase | | Toluidine Blue O. |
| Issue Date: | Feb-2004 |
| Publisher: | Blackwell Publishing |
| Journal Title: | New Phytologist |
| Volume: | 161 |
| Issue: | 2 |
| Start Page: | 387 |
| End Page: | 392 |
| Publisher DOI: | 10.1046/j.1469-8137.2003.00966.x |
| Abstract: | The rate of polyphosphate accumulation in extraradical hyphae of an arbuscular mycorrhizal fungus was investigated by conventional histochemistry and a new enzymatic method using a bacterial enzyme, polyphosphate kinase. Marigold ( Tagetes patula cv. Bonanza Orange) was inoculated with Archaeospora leptoticha and grown under P-deficient conditions. Extraradical hyphae were harvested at 0, 1, 3 and 24 h after 1 m M P-application. PolyP levels were assessed by both metachromasy of Toluidine blue O and polyphosphate kinase which converted polyP to ATP followed by the ATP-luciferase assay. Percentage of hyphae with metachromatic granules was increased from 25 to 44% from 0 to 1 h, and a maximum of 50% was reach by 3 h. Polyphosphate content was doubled from 1 to 3 h after P-application (4.8–10.0 μmol as Pi mg − 1 protein) at a rate of 46.4 ± 15.1 nmol min − 1 mg − 1 . The rate of polyphosphate accumulation in the hyphae was surprisingly rapid as those of polyphosphate-hyper accumulating microorganisms. The enzymatic method employed in the present study allows highly specific and sensitive assessment of polyphosphate in the mycorrhizal system. |
| Description: | The definitive version is available at www.blackwellsynergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/932 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 江澤 辰広
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