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Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length

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タイトル: Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length
著者: Nakahara, Masaki 著作を一覧する
Takagi, Mutsumi 著作を一覧する
Hattori, Takako 著作を一覧する
Wakitani, Shigeyuki 著作を一覧する
Yoshida, Toshiomi 著作を一覧する
キーワード: Chondrogenesis
Hematopoiesis
Mesenchymal stem cell
Population doubling level
Subcultivation
Telomere
発行日: 2005年 1月
出版者: Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
誌名: Cytotechnology
巻: 47
号: 1-3
開始ページ: 19
終了ページ: 27
出版社 DOI: 10.1007/s10616-005-3752-9
抄録: Effects of subcultivation of human bone marrow mesenchymal stem cells on their capacities for chondrogenesis and supporting hematopoiesis, and telomea length were investigated. Mesenchymal stem cells were isolated from human bone marrow aspirates and subcultivated several times at 37℃ under a 5% CO2 atmosphere employing DMEM medium containing 10% FCS up to the 20th population doubling level (PDL). The ratio of CD45- CD105+ cells among these cells slightly increased as PDL increased. However, there was no marked change in the chondrogenic capacity of these cells, which was confirmed by expression assay of aggrecan mRNA and Safranin O staining after pellet cell cultivation. The change in capacity to support hematopoiesis of cord blood cells was not observed among cells with various PDLs. On the other hand, telomere length markedly decreased as PDL increased at a higher rate than that at which telomere length of primary mesenchymal stem cells decreased as the age of donor increased.
Rights: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/933
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高木 睦

 

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