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Hokkaido University Collection of Scholarly and Academic Papers >
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Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins
| Title: | Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins |
| Authors: | Rajapakse, Sanath Browse this author | | Ogiwara, Katsueki Browse this author | | Takano, Naoharu Browse this author | | Moriyama, Akihiko Browse this author | | Takahashi, Takayuki5 Browse this author →KAKEN DB |
| Authors(alt): | 高橋, 孝行5 |
| Keywords: | Human kallikrein 8 | | Enzymatic characterization | | Extracellular matrix proteins | | Tissue-type plasminogen activator |
| Issue Date: | 1-Dec-2005 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Febs Letters |
| Volume: | 579 |
| Issue: | 30 |
| Start Page: | 6879 |
| End Page: | 6884 |
| Publisher DOI: | 10.1016/j.febslet.2005.11.039 |
| PMID: | 16337200 |
| Abstract: | Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and enzymatic characterizations. hK8 had trypsin-like activity with a strong preference for Arg over Lys in the P1 position, and its activity was inhibited by typical serine protease inhibitors. The protease degraded casein, fibronectin, gelatin, collagen type IV, fibrinogen, and high-molecular-weight kininogen. hK8 also converted human single-chain tissue-type plasminogen activator (65 kDa) to its two-chain form (32 and 33 kDa) by specifically cleaving the peptide bond Arg275–Ile276. This conversion resulted in a drastic increase in the activity of the activator toward the fluorogenic substrate Pyr-Gly-Arg-MCA and plasminogen in the absence of fibrin. Our findings suggest that hK8 may be implicated in ECM protein degradation in the area surrounding hK8-producing cells. |
| Relation: | http://www.sciencedirect.com/science/journal/00145793 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/985 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荻原 克益
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