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Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins

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Title: Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins
Authors: Rajapakse, Sanath Browse this author
Ogiwara, Katsueki Browse this author
Takano, Naoharu Browse this author
Moriyama, Akihiko Browse this author
Takahashi, Takayuki5 Browse this author →KAKEN DB
Authors(alt): 高橋, 孝行5
Keywords: Human kallikrein 8
Enzymatic characterization
Extracellular matrix proteins
Tissue-type plasminogen activator
Issue Date: 1-Dec-2005
Publisher: Elsevier B.V.
Journal Title: Febs Letters
Volume: 579
Issue: 30
Start Page: 6879
End Page: 6884
Publisher DOI: 10.1016/j.febslet.2005.11.039
PMID: 16337200
Abstract: Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and enzymatic characterizations. hK8 had trypsin-like activity with a strong preference for Arg over Lys in the P1 position, and its activity was inhibited by typical serine protease inhibitors. The protease degraded casein, fibronectin, gelatin, collagen type IV, fibrinogen, and high-molecular-weight kininogen. hK8 also converted human single-chain tissue-type plasminogen activator (65 kDa) to its two-chain form (32 and 33 kDa) by specifically cleaving the peptide bond Arg275–Ile276. This conversion resulted in a drastic increase in the activity of the activator toward the fluorogenic substrate Pyr-Gly-Arg-MCA and plasminogen in the absence of fibrin. Our findings suggest that hK8 may be implicated in ECM protein degradation in the area surrounding hK8-producing cells.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 孝行

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