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Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing

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Title: Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing
Authors: Hisano, Hiroshi Browse this author
Kanazawa, Akira Browse this author →KAKEN DB
Kawakami, Akira Browse this author
Yoshida, Midori Browse this author
Shimamoto, Yoshiya Browse this author
Yamada, Toshihiko6 Browse this author →KAKEN DB
Authors(alt): 山田, 敏彦6
Keywords: Freezing tolerance
Fructan
Lolium perenne
Transgenic plants
Issue Date: 14-Jul-2004
Publisher: Elsevier Ireland Ltd.
Journal Title: Plant Science
Volume: 167
Issue: 4
Start Page: 861
End Page: 868
Publisher DOI: 10.1016/j.plantsci.2004.05.037
Abstract: The accumulation of fructan in grasses during autumn is linked to winter hardiness. Genetic manipulation of the accumulation of fructan could be an important molecular breeding strategy for the improvement of winter hardiness in grasses. We produced transgenic perennial ryegrass (Lolium perenne) plants that overexpress wheat fructosyltransferase genes, wft1 and wft2, which encode sucrose–fructan 6-fructosyltransferase (6-SFT) and sucrose–sucrose 1-fructosyltransferase (1-SST), respectively, under the control of CaMV 35S promoter using a particle bombardment-mediated method of transformation. Significant increases in fructan content were detected in the transgenic perennial ryegrass plants. A freezing test using the electrical conductivity method indicated that transgenic plants that accumulated a greater amount of fructan than non-transgenic plants have increased tolerance on a cellular level to freezing. The results suggest that the overexpression of the genes involved in fructan synthesis serves as a novel strategy to produce freezing-tolerant grasses.
Relation: http://www.sciencedirect.com/science/journal/01689452
Type: article (author version)
URI: http://hdl.handle.net/2115/993
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 敏彦

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