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Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars

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Title: Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars
Authors: Guo, Yangdong Browse this author
Mizukami, Yuko Browse this author
Yamada, Toshihiko3 Browse this author →KAKEN DB
Authors(alt): 山田, 敏彦3
Keywords: Androgenesis
Forage grass
freezing tolerance
Genome size
Genomic in situ hybridisation (GISH)
pollen fertility
Issue Date: 2005
Publisher: Blackwell Science Ltd
Journal Title: New Phytologist
Volume: 166
Issue: 2
Start Page: 455
End Page: 464
Publisher DOI: 10.1111/j.1469-8137.2005.01345.x
Abstract: A successful androgenesis in amphidiploid Festulolium (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) was obtained using PG-96 medium for embryo/callus induction. The green plant regeneration varied, and was 46 %, 35 % and 17 % for Bx350, Bx351 and Prior, respectively and over 800 green plants have been obtained. Androgenic progeny showed a large variation in freezing tolerance, 7 % of 292 progeny exceeding that of freezing hardy F. pratensis despite containing chromosomes of L. perenne, a more freezing-sensitive species. More than 60% of flowering 175 progeny produced dehiscent anthers with pollen stainability ranging from 5% to 85%. Androgenic plants contained 14 or 28 chromosomes. There were 188 (56 %), 204 (77 %) and 114 dihaploids (81 %) from Bx350, Bx351 and Prior, respectively. However, the nuclear DNA content varied significantly even between plants with the same chromosome number. Variation in DNA content reflected the genetic variation inherent in androgenic populations. High levels of chromosome pairing and recombination were observed due to close homology between genomes of L. perenne and F. pratensis.
Description: The definitive version is available at
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 敏彦

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