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Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars
| Title: | Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars |
| Authors: | Guo, Yangdong Browse this author | | Mizukami, Yuko Browse this author | | Yamada, Toshihiko3 Browse this author →KAKEN DB |
| Authors(alt): | 山田, 敏彦3 |
| Keywords: | Androgenesis | | Festulolium | | Forage grass | | freezing tolerance | | Genome size | | Genomic in situ hybridisation (GISH) | | pollen fertility |
| Issue Date: | 2005 |
| Publisher: | Blackwell Science Ltd |
| Journal Title: | New Phytologist |
| Volume: | 166 |
| Issue: | 2 |
| Start Page: | 455 |
| End Page: | 464 |
| Publisher DOI: | 10.1111/j.1469-8137.2005.01345.x |
| Abstract: | A successful androgenesis in amphidiploid Festulolium (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) was obtained using PG-96 medium for embryo/callus induction. The green plant regeneration varied, and was 46 %, 35 % and 17 % for Bx350, Bx351 and Prior, respectively and over 800 green plants have been obtained. Androgenic progeny showed a large variation in freezing tolerance, 7 % of 292 progeny exceeding that of freezing hardy F. pratensis despite containing chromosomes of L. perenne, a more freezing-sensitive species. More than 60% of flowering 175 progeny produced dehiscent anthers with pollen stainability ranging from 5% to 85%.
Androgenic plants contained 14 or 28 chromosomes. There were 188 (56 %), 204 (77 %) and 114 dihaploids (81 %) from Bx350, Bx351 and Prior, respectively. However, the nuclear DNA content varied significantly even between plants with the same chromosome number. Variation in DNA content reflected the genetic variation inherent in androgenic populations.
High levels of chromosome pairing and recombination were observed due to close homology between genomes of L. perenne and F. pratensis. |
| Description: | The definitive version is available at www.blackwell-synergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/998 |
| Appears in Collections: | 北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 敏彦
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