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Identification of cultivars and accessions of Lolium, Festuca and Festulolium hybrids through the detection of simple sequence repeat (SSR) polymorphism

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Title: Identification of cultivars and accessions of Lolium, Festuca and Festulolium hybrids through the detection of simple sequence repeat (SSR) polymorphism
Authors: Momotaz, Aliya Browse this author
Forster, John W. Browse this author
Yamada, Toshihiko3 Browse this author →KAKEN DB
Authors(alt): 山田, 敏彦3
Keywords: Festuca arundinacea
F. pratensis
Lolium multiflorum
L. perenne
Lolium/Festuca complex
genetic diversity
SSR marker
Issue Date: 2003
Publisher: Blackwell Verlag
Journal Title: Plant Breeding
Volume: 123
Issue: 4
Start Page: 370
End Page: 376
Publisher DOI: 10.1111/j.1439-0523.2004.00962.x
Abstract: Molecular diversity and genetic affinity in the Lolium/Festuca grass complex have been assessed using simple sequence repeat (SSR) marker technology. The genotypic set was derived from three accessions of Lolium perenne, two cultivars of Lolium multiflorum, two cultivars of Festuca pratensis, two cultivars of Festuca arunidinacea and ten accessions from different intergeneric hybrid (Festulolium) combinations. The majority of the genomic DNA-derived SSR primer pairs from perennial ryegrass (LPSSR) and Italian ryegrass (LMSSR) produced clear, simple and distinctive amplification products from the majority of the genotypes. The efficiency of cross-specific amplification for LPSSR markers varied from 38% in F. pratensis to 93% in two cultivars of Festulolium and for LMSSR markers from 57% in F. pratensis to 87% in L. multiflorum. Of 40 amplified markers, fourteen (35%) produced species-difference alleles in the relation to cultivars used in the present study. Thirty-five LPSSR locus-derived alleles were found to be specific to Lolium species, 4 to F. pratensis and 6 to F. arundinacea. For LMSSR alleles, 8 were specific to Lolium species and 5 were only associated with L. multiflorum, and null alleles were detected for F. pratensis in all instances. These species-difference markers could clearly identify different accessions of Festulolium. Cluster analysis separated the individual taxa and showed grouping of intergeneric hybrids based on genomic composition. The data distinguished between the species and reflected the known pedigree of the cultivars and the differences between the species. The dendrogram also distinguished between the Festulolium accessions and clearly demonstrated the relations between Festulolium hybrids and their parent species.
Description: The definitive version is available at
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 敏彦

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