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Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells
| Title: | Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells |
| Authors: | Satou, Kazuya Browse this author | | Kawai, Kazuaki Browse this author | | Kasai, Hiroshi Browse this author | | Harashima, Hideyoshi Browse this author →KAKEN DB | | Kamiya, Hiroyuki Browse this author |
| Keywords: | Reactive oxygen species | | 8-Hydroxy-dGTP | | Mutation | | Mammalian cells |
| Issue Date: | 15-May-2007 |
| Publisher: | Elsevier |
| Journal Title: | Free Radical Biology and Medicine |
| Volume: | 42 |
| Issue: | 10 |
| Start Page: | 1552 |
| End Page: | 1560 |
| Publisher DOI: | 10.1016/j.freeradbiomed.2007.02.024 |
| PMID: | 17448902 |
| Abstract: | The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by means of cationic liposomes, and the DNAs replicated in the cells were recovered and then transfected into Escherichia coli. 8-OH-dGTP induced A:T->C:G substitution mutations in the COS-7 cells. This result agrees with previous observations indicating that DNA polymerases misincorporate 8-OH-dGTP opposite A in vitro, and that the oxidized deoxyribonucleotide induces A:T->C:G transversions in E. coli. These results constitute the first direct evidence to show that 8-OH-dGTP actually induces mutations in living mammalian cells. |
| Relation: | http://www.sciencedirect.com/science/journal/08915849 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/20538 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 紙谷 浩之
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