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Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells

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Title: Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells
Authors: Satou, Kazuya Browse this author
Kawai, Kazuaki Browse this author
Kasai, Hiroshi Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Kamiya, Hiroyuki Browse this author
Keywords: Reactive oxygen species
Mammalian cells
Issue Date: 15-May-2007
Publisher: Elsevier
Journal Title: Free Radical Biology and Medicine
Volume: 42
Issue: 10
Start Page: 1552
End Page: 1560
Publisher DOI: 10.1016/j.freeradbiomed.2007.02.024
PMID: 17448902
Abstract: The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by means of cationic liposomes, and the DNAs replicated in the cells were recovered and then transfected into Escherichia coli. 8-OH-dGTP induced A:T->C:G substitution mutations in the COS-7 cells. This result agrees with previous observations indicating that DNA polymerases misincorporate 8-OH-dGTP opposite A in vitro, and that the oxidized deoxyribonucleotide induces A:T->C:G transversions in E. coli. These results constitute the first direct evidence to show that 8-OH-dGTP actually induces mutations in living mammalian cells.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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