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Hokkaido University Collection of Scholarly and Academic Papers >
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Peer-reviewed Journal Articles, etc >
LIF- and IL-6-Induced Acetylation of STAT3 at Lys-685 through PI3K/Akt Activation.
| Title: | LIF- and IL-6-Induced Acetylation of STAT3 at Lys-685 through PI3K/Akt Activation. |
| Authors: | Ohbayashi, Norihiko Browse this author | | Ikeda, Osamu Browse this author | | Taira, Naohisa Browse this author | | Yamamoto, Yu Browse this author | | Muromoto, Ryuta Browse this author | | Sekine, Yuichi Browse this author | | Sugiyama, Kenji Browse this author | | Honjoh, Tsutomu Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | signal transducer and activator of transcription 3 | | interleukin-6 | | acetylation | | leukemia inhibitory factor | | PI3K | | Akt |
| Issue Date: | Oct-2007 |
| Publisher: | 日本薬学会 |
| Journal Title: | Biological & Pharmaceutical Bulletin |
| Volume: | 30 |
| Issue: | 10 |
| Start Page: | 1860 |
| End Page: | 1864 |
| Publisher DOI: | 10.1248/bpb.30.1860 |
| PMID: | 17917251 |
| Abstract: | Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. A recent study has demonstrated, by using antibody to acetylated lysine, and a STAT3 mutant with Lys-685-to-Arg substitution, that STAT3 is acetylated at Lys-685 by histone acetyltransferase p300, and that acetylation at Lys-685 is critical for STAT3 activation. In the present study, we created an acetyl-specific antibody against STAT3 acetylated at Lys-685, and found that leukemia inhibitory factor (LIF) or interleukin (IL)-6 induced acetylation of STAT3 at Lys-685 in 293T and Hep3B cells. Moreover, acetylation of STAT3 at Lys-685 was suppressed by PI3K inhibitor LY294002, or a dominant negative Akt. Taken together, our findings demonstrate that endogenous STAT3 is acetylated at Lys-685 by LIF or IL-6 through PI3K/Akt activation. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/28780 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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