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LIF- and IL-6-Induced Acetylation of STAT3 at Lys-685 through PI3K/Akt Activation.

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Title: LIF- and IL-6-Induced Acetylation of STAT3 at Lys-685 through PI3K/Akt Activation.
Authors: Ohbayashi, Norihiko Browse this author
Ikeda, Osamu Browse this author
Taira, Naohisa Browse this author
Yamamoto, Yu Browse this author
Muromoto, Ryuta Browse this author
Sekine, Yuichi Browse this author
Sugiyama, Kenji Browse this author
Honjoh, Tsutomu Browse this author
Matsuda, Tadashi Browse this author →KAKEN DB
Keywords: signal transducer and activator of transcription 3
interleukin-6
acetylation
leukemia inhibitory factor
PI3K
Akt
Issue Date: Oct-2007
Publisher: 日本薬学会
Journal Title: Biological & Pharmaceutical Bulletin
Volume: 30
Issue: 10
Start Page: 1860
End Page: 1864
Publisher DOI: 10.1248/bpb.30.1860
PMID: 17917251
Abstract: Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. A recent study has demonstrated, by using antibody to acetylated lysine, and a STAT3 mutant with Lys-685-to-Arg substitution, that STAT3 is acetylated at Lys-685 by histone acetyltransferase p300, and that acetylation at Lys-685 is critical for STAT3 activation. In the present study, we created an acetyl-specific antibody against STAT3 acetylated at Lys-685, and found that leukemia inhibitory factor (LIF) or interleukin (IL)-6 induced acetylation of STAT3 at Lys-685 in 293T and Hep3B cells. Moreover, acetylation of STAT3 at Lys-685 was suppressed by PI3K inhibitor LY294002, or a dominant negative Akt. Taken together, our findings demonstrate that endogenous STAT3 is acetylated at Lys-685 by LIF or IL-6 through PI3K/Akt activation.
Type: article
URI: http://hdl.handle.net/2115/28780
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松田 正

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