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Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases
Title: | Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases |
Other Titles: | Incorporation of 8-hydroxyguanosine 5'-triphosphate (8-oxo-7,8-dihydroguanosine 5'-triphosphate) by bacterial and human RNA polymerases |
Authors: | Kamiya, Hiroyuki Browse this author | Suzuki, Akihiro Browse this author | Yamaguchi, Yuki Browse this author | Handa, Hiroshi Browse this author | Harashima, Hideyoshi Browse this author →KAKEN DB |
Keywords: | Oxidized ribonucleotide | 8-Hydroxyguanosine 5'-triphosphate | RNA polymerase | Transcription | Free radicals |
Issue Date: | 15-Jun-2009 |
Publisher: | Elsevier Inc. |
Journal Title: | Free Radical Biology and Medicine |
Volume: | 46 |
Issue: | 12 |
Start Page: | 1703 |
End Page: | 1707 |
Publisher DOI: | 10.1016/j.freeradbiomed.2009.04.005 |
PMID: | 19362141 |
Abstract: | Oxidized RNA precursors formed in the nucleotide pool may be incorporated into RNA. In this study, the incorporation of 8-hydroxyguanosine 5'-triphosphate (8-OH-GTP; 8-oxo-7,8-dihydroguanosine 5'-triphosphate) into RNA by Escherichia coli RNA polymerase was examined in vitro, using a primer RNA and a template DNA with defined sequences. 8-OH-GTP was incorporated opposite C and A in the template DNA. Surprisingly, 8-OH-GTP was quite efficiently incorporated by the bacterial RNA polymerase, in contrast to the incorporation of the 2'-deoxyribo-counterpart by DNA polymerases, as indicated by the kinetic parameters. The primer was further extended by the addition of a ribonucleotide complementary to the nucleobase adjacent to C or A (the nucleobase opposite which 8-OH-GTP was inserted). Thus, the incorporation of 8-OH-GTP did not completely inhibit further RNA chain elongation. 8-OH-GTP was also incorporated opposite C and A by human RNA polymerase II. These results suggest that 8-OH-GTP in the nucleotide pool call cause the formation of oxidized RNA and disturb the transmittance of genetic information. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/38630 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 紙谷 浩之
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