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Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens

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Title: Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens
Authors: Guo, Yang-Dong Browse this author
Hisano, Hiroshi Browse this author
Shimamoto, Yoshiya Browse this author
Yamada, Toshihiko Browse this author →KAKEN DB
Keywords: androgenic callus
anther culture
dihaploid
GUS activity
transgenic forage grass
Issue Date: Feb-2009
Publisher: Springer
Journal Title: Plant Cell, Tissue and Organ Culture
Volume: 96
Issue: 2
Start Page: 219
End Page: 227
Publisher DOI: 10.1007/s11240-008-9478-6
Abstract: Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgenes was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n=2x=14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/42593
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 敏彦

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