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Hokkaido University Collection of Scholarly and Academic Papers >
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Peer-reviewed Journal Articles, etc >
Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens
| Title: | Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens |
| Authors: | Guo, Yang-Dong Browse this author | | Hisano, Hiroshi Browse this author | | Shimamoto, Yoshiya Browse this author | | Yamada, Toshihiko Browse this author →KAKEN DB |
| Keywords: | androgenic callus | | anther culture | | dihaploid | | GUS activity | | transgenic forage grass |
| Issue Date: | Feb-2009 |
| Publisher: | Springer |
| Journal Title: | Plant Cell, Tissue and Organ Culture |
| Volume: | 96 |
| Issue: | 2 |
| Start Page: | 219 |
| End Page: | 227 |
| Publisher DOI: | 10.1007/s11240-008-9478-6 |
| Abstract: | Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgenes was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n=2x=14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/42593 |
| Appears in Collections: | 北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 敏彦
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