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Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins
| Title: | Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins |
| Authors: | Matsumoto, Ken'ichiro Browse this author →KAKEN DB | | Orikasa, Yoshitake Browse this author | | Ichinohe, Kenta Browse this author | | Hashimoto, Shigeki Browse this author | | Ooi, Toshihiko Browse this author →KAKEN DB | | Taguchi, Seiichi Browse this author →KAKEN DB |
| Keywords: | Cell penetration | | Flow cytometry | | Antibacterial peptide | | Honey bee |
| Issue Date: | 23-Apr-2010 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 395 |
| Issue: | 1 |
| Start Page: | 7 |
| End Page: | 10 |
| Publisher DOI: | 10.1016/j.bbrc.2010.03.088 |
| PMID: | 20302844 |
| Abstract: | Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43104 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松本 謙一郎
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