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Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins

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Title: Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecins
Authors: Matsumoto, Ken'ichiro Browse this author →KAKEN DB
Orikasa, Yoshitake Browse this author
Ichinohe, Kenta Browse this author
Hashimoto, Shigeki Browse this author
Ooi, Toshihiko Browse this author →KAKEN DB
Taguchi, Seiichi Browse this author →KAKEN DB
Keywords: Cell penetration
Flow cytometry
Antibacterial peptide
Honey bee
Issue Date: 23-Apr-2010
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 395
Issue: 1
Start Page: 7
End Page: 10
Publisher DOI: 10.1016/j.bbrc.2010.03.088
PMID: 20302844
Abstract: Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency.
Type: article (author version)
URI: http://hdl.handle.net/2115/43104
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松本 謙一郎

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